{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:58:29Z","timestamp":1790917109615,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":7215,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1986,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leukaemic cells from 2 patients with B\u2010prolymphocytic leukaemia were immortalized <jats:italic>in vitro<\/jats:italic> by means of Epstein\u2010Barr virus and phorbol\u2010ester TPA. The resulting cell lines, named JVM\u20102 and JVM\u20103, have been growing continuously in liquid culture for more than one year. JVM\u20102 is characterized cytogenetically by t(11;14)(q13;q32), and JVM\u20103 by trisomy 12. The immunoglobulin (Ig) heavy\u2010and light\u2010chain genes showed the same pattern of rearrangement in both lines as in the original prolymphocytes from each case. The cells from these lines showed a spectrum of morphological and immunological features corresponding to different stages of B\u2010cell maturation. The expression of Ig, IgM\u2010X in JVM\u20102 and IgMD\u2010K in JVM\u20103, changed from a predominantly membrane pattern in the original cells to a cytoplasmic one in the cell lines. By comparison with their original progenitors, the cells from both lines showed reduced reactivity with the monoclonal antibody (MAb) FMC7, and increased expression of the antigens recognized by the MAbs OKT10, \u03b1\u2010Tac, FMC53 and Ki\u2010I. The availability of cell lines from this rare type of lymphoid leukaemia offers a potential tool for the study of molecular events associated with the expression of Ig and other antigens by ncoplastic cells.<\/jats:p>","DOI":"10.1002\/ijc.2910380413","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T20:17:52Z","timestamp":1171916272000},"page":"531-538","source":"Crossref","is-referenced-by-count":51,"title":["Two new cell lines from B\u2010prolymphocytic leukaemia: Characterization by morphology, immunological markers, karyotype and Ig gene rearrangement"],"prefix":"10.1002","volume":"38","author":[{"given":"Junia V.","family":"Melo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasantha","family":"Brito\u2010Babapulle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Letizia","family":"Foroni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele S. F.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucio","family":"Luzzatto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Catovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90508-1"},{"key":"e_1_2_1_3_1","unstructured":"Brito\u2010Babapulle V. Pittman S. Melo J. V. Pomfret M.andCatovsky D. Cytogenetic studies in prolymphocytic leikemia. I. 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