{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:27:04Z","timestamp":1756996024181},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":6942,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1987,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>T4 subpopulation of T lymphocytes is the preferential target of infection with human T leukemia\/lymphoma virus of subgroup I (HTLV\u2010I). In this study we attempt to determine whether different T\u2010cell subsets exbibit differences in susceptibility to virus infection. T cells from cord or peripheral blood were separated according to cell densities and T\u2010cell surface markers by Percoll gradient and Sepharose anti\u2010Fab immunoadsorbent affinity column (IAC), respectively. Separated T\u2010cell subpopulations were infected with HTLV\u2010I, by means of co\u2010cultivation with irradiated virus producer cell lines (MT\u20102, TK). Percentages of HTLV\u2010I\u2010infected cells were assayed by immunofluorescence assay (IFA), using highly specific mouse monoclonal antibody (MAb) directed against HTLV\u2010I p19 core protein. The results showed that different T\u2010cell subpopulations separated either by Percoll or by IAC were susceptible to HTLV\u2010I infection with the exception of large granular lymphocytes (LGL), which exhibit high cell\u2010mediated natural cytotoxicity (CMNC). The susceptibility to HTLV\u2010I infection of T cells with CMNC activity was further studied on established cell clones with LGL morphology. The results showed again that these cells were resistant to the virus infection. The present studies indicate that different T\u2010cell subpopulations, irrespective of their size and of cell\u2010surface markers, are susceptible to HTLV\u2010I infection, with the exception of functionally mature LGL or of immortalized LGL clones.<\/jats:p>","DOI":"10.1002\/ijc.2910400102","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T20:54:42Z","timestamp":1171918482000},"page":"1-6","source":"Crossref","is-referenced-by-count":10,"title":["<i>In vitro<\/i> susceptibility of different human T\u2010cell subpopulations and resistance of large granular lymphocytes to HTLV\u2010I infection"],"prefix":"10.1002","volume":"40","author":[{"given":"B.","family":"Macchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Popovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Allavena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Ortaldo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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