{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T04:40:46Z","timestamp":1725684046759},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":6699,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1988,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The activity of ecto\u2010nucleotidases has been determined on intact cells from Burkitt lymphoma (BL) and lymphoblastoid cell lines established <jats:italic>in vitro<\/jats:italic> (LCLs). BL cells never express ecto\u20105\u2032\u2010nudeotidase (5\u2032\u2010N) and exhibit only low levels of ecto\u2010ATPase activity, whereas LCL\u2010cells usually express both enzymes to varying degrees. There is a certain correlation (r = 0.75) between 5\u2032\u2010N and ecto\u2010ATPase. When cAMP formation in response to agonists of the A2\u2010typc (stimulating) adenosine receptor is measured in the same cell lines there is no correlation with the expression of ecto\u2010nucleotidases. Within pairs of BL and LCL cell lines derived from the same donor, 5\u2032\u2010N inverse relationship between ecto\u2010nucleotidases and the response to the adenosine receptor agonist N\u2010ethyl\u2010car\u2010boxamido\u2010adenosine (NECA) is observed. BL cells show a good response to NECA, whereas this is low or absent in LCL cells. Treatment of cells which exhibit both S\u2032\u2010N and the adenosine receptor with specific polyclonal or monoclonal antibodies against the enzyme does not impair the function of the receptor. Antisera against peptides of the membrane antigen BNLF I\u2010MA, coded by the EBV\u2010genome, do not co\u2010precipitate 5\u2032\u2010N out of detergent extracts of LCL\u2010cells. In both cases 5\u2032\u2010N cannot be closely associated with other membrane components. The differences between BL and LCL cells in ecto\u2010nucleotidases and the adenosine receptor appear to be fairly stable phenotypical markers, independent of other parameters and suitable for use in distinguishing these cells in all populations.<\/jats:p>","DOI":"10.1002\/ijc.2910410321","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:01:09Z","timestamp":1171918869000},"page":"436-441","source":"Crossref","is-referenced-by-count":4,"title":["Qualitative and quantitative differences between Burkitt lymphoma and lymphoblastoid cell lines in the expression of membrane ecto\u2010nucleotidases and in the response to agonists of the A<sub>2<\/sub>\u2010type adenosine receptor"],"prefix":"10.1002","volume":"41","author":[{"given":"Wolf","family":"Gutensohn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidi","family":"Jahn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-2985(87)80030-X"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.4049\/jimmunol.132.4.1767","article-title":"Lymphocyte ecto\u20105\u2032\u2010nucleotidase activity in infancy: Increasing activity blood B cells precedes their ability to synthesize IgG in vitro","volume":"132","author":"Bastian J. 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An explanation for the heterogeneity in B lymphocyte ecto\u20105\u2032\u2010nucleotidase activity in patients with hypogamma\u2010globulinemia","volume":"137","author":"Thompson L. F.","year":"1986","journal-title":"J. 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