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The present study shows that the sub\u2010fragment containing the central open reading frame BERF2b of the BamHI E fragment (Baer <jats:italic>et al.<\/jats:italic>, 1984) is responsible for nuclear antigen induction. In addition, 2 fragments corresponding to 2 other open reading frames of the BamHI E, BERF1 and BERF4 also induce nuclear antigens upon transfection into BHK cells. These 3 antigens, designated RF2b, RF1 and RF4 antigens, were serologically classified as EBNA and andgenically distinct. In immunoblotting analysis of latently EBV\u2010infected BJ\u2010B95\u20108 cells, 3 high\u2010molecular\u2010weight polypeptides (136, 142 and 147 kDa) ware identified by anti\u2010EBNA sera. Immunoblotting analysis of transfected BHK cells indicated that the RF2b antigen is 145 kDa in its native form and antigenically related to the 147\u2010kDa protein of BJ\u2010B95\u2010B cells. Although RF1 and RF4 antigens were not detected by immunoblotting, reactivities of sera with RF1 and RF4 antigens in the immunofluorescence test were correlated with those of sera with the 136\u2010 and 142\u2010kDa polypeptides of BJ\u2010B95\u20108 cells, respectively. The results suggest that 3 high\u2010molecular\u2010weight proteins of latently EBV\u2010infected cells are encoded by 3 open reading frames of the BamHI E DNA fragment.<\/jats:p>","DOI":"10.1002\/ijc.2910410518","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:20:20Z","timestamp":1171920020000},"page":"744-751","source":"Crossref","is-referenced-by-count":19,"title":["Three epstein\u2010barr virus (EBV)\u2010determined nuclear antigens induced by the BamHI E region of EBV DNA"],"prefix":"10.1002","volume":"41","author":[{"given":"Norio","family":"Shimizu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuo","family":"Yamaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadatoshi","family":"Sakuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Ono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenzo","family":"Takada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/310207a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.17.7103"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90281-5"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/jvi.53.2.515-521.1985","article-title":"Transcriptional activity of avian retroviral long terminal repeats directly correlates with enhancer activity","volume":"53","author":"Cullen B. 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