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The anti\u2010CD20 (Bp35) MAb IF5 can deliver a strong activation signal to resting normal B cells, and the anti\u2010CDw40 (Bp50) MAb G28\u20105 can promote activated G<jats:sub>1<\/jats:sub> B cells to enter S phase. These antibodies were tested for their functional effects <jats:italic>in vitro<\/jats:italic> on suspended cells from 17 follicle\u2010center\u2010cell (FCC) lymphomas, 5 cases of chronic lymphatic B\u2010cell leukemia (B\u2010CLL) and 8 cases of various histological types. Changes in cellular volume, RNA and DNA synthesis were compared with the results obtained with a polyclonal anti\u2010\u03bc [F(ab\u2032)<jats:sub>2<\/jats:sub>] antiserum, a MAb to surface IgM (AF6), 12\u2010O\u2010tetradecanoyl\u2010phorbol\u2010 13\u2010acetate (TPA) and B\u2010cell growth factor (low\u2010molecular\u2010weight BCGF). Our data reveal differences in the requirements for triggering of various B\u2010cell subsets: cells from CLL responded strongly to TPA but not to anti\u2010\u03bc, which is a potent stimulator not only of normal B cells but also of cells from individual cases of FCC lymphomas. Our observations suggest that the differentiation stage of B\u2010CLL cells is distinct from that of small resting B cells from peripheral blood. Centrocytic lymphomas could not be activated by any of the reagents. CD20\u2010mediated triggering was seen in neoplastic B cells from only 4 of 30 cases, Indicating that most B\u2010cell neoplasias were not responsive to this activation pathway. In contrast, the anti\u2010CDw40 MAb consistently stimulated DNA synthesis together with anti\u2010\u03bc or TPA in cells from FCC lymphomas, but not from CLL. Together, these results suggest that activation in different neoplastic B\u2010cell subsets depends on distinct signal transduction mechanisms.<\/jats:p>","DOI":"10.1002\/ijc.2910420409","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:13:49Z","timestamp":1171919629000},"page":"521-528","source":"Crossref","is-referenced-by-count":15,"title":["Triggering of neoplastic B cells <i>via<\/i> surface IgM and the cell surface antigens CD20 and CDw40. Responses differ from normal blood B cells and are restricted to certain morphologic subsets"],"prefix":"10.1002","volume":"42","author":[{"given":"Klaus","family":"Beiske","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward A.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Holte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey A.","family":"Ledbetter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erlend B.","family":"Smeland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tore","family":"Godal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(73)90167-8"},{"key":"e_1_2_1_3_1","first-page":"362","article-title":"Induction of maturation of human B\u2010Cell lymphomas in vitro","volume":"115","author":"Beiske K.","year":"1984","journal-title":"Amer. 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