{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T01:20:53Z","timestamp":1700702453970},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":6454,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1988,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Epstein\u2010Barr virus (EBV) nuclear antigen 2 (EBNA\u20102) is the only one of the EBNA proteins to have been implicated as an EBV\u2010encoded transforming protein. More detailed studies of this protein have been hampered by the lack of EBNA\u20102\u2010specific monoclonal antibodies (MAbs) and of purified protein. To overcome these problems, we isolated 5 hybridomas producing MAbs reactive with an 18 residue synthetic peptide corresponding to the carboxyterminus of EBNA\u20102. Four of the 5 MAbs were specifically reactive with EBNA\u20102 in its denatured form on immunoblots. The 5th antibody (IISE) was reactive with the native form of EBNA\u20102. By using a one\u2010step immunoaffinity purification method with II5E cross\u2010linked to protein\u2010A\u2010Sepharose, we purified EBNA\u20102 to homogeneity, i. e., more than 1,200\u2010fold, from Burkitt lymphoma cell extracts. A major 32\u2010kDa associated protein and a less abundant 17\u2010kDa protein were co\u2010purified with EBNA\u20102. Immunoprecipitation with 115E from <jats:sup>35<\/jats:sup>S\u2010methionine\u2010labelled cell extracts showed that the 32\u2010kDa protein co\u2010precipitated with EBNA\u20102 from EBV\u2010positive cells, but was not detectable in immunoprecipitates of EBV\u2010negative cells. When the immunoprecipitates or the purified proteins were immunoblotted with EBV\u2010immune sera, only EBNA\u20102 was reactive, indicating that the associated proteins are of cellular origin. Immunoprecipitation of cells labelled with <jats:sup>32<\/jats:sup>P\u2010orthophosphate showed that EBNA\u20102, but not the associated proteins, is a phosphoprotein. The expression level of EBNA\u20102 varied between different EBV\u2010carrying cell lines, as measured by a 2\u2010site ELISA based on antibody II5E. In indirect immunofluorescence, the II5E MAb gave an EBNA\u20102\u2010specific characteristic granular staining pattern. These characteristics of EBNA\u20102 resemble those of other viral transforming proteins.<\/jats:p>","DOI":"10.1002\/ijc.2910420516","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:13:22Z","timestamp":1171919602000},"page":"721-727","source":"Crossref","is-referenced-by-count":15,"title":["Purification and characterization of the epstein\u2010barr virus nuclear antigen 2 using monoclonal antipeptide antibodies"],"prefix":"10.1002","volume":"42","author":[{"given":"Joakim","family":"Dillner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vidar","family":"Wendel\u2010Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunilla","family":"Kjellstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengt","family":"Kallin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Ros\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(85)90253-3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.59.2.453-462.1986"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1128\/jvi.27.1.81-89.1978","article-title":"Heterogeneity of Epstein\u2010Barr virus. 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