{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:57:17Z","timestamp":1761058637959},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":6272,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1989,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immunoglobulin heavy\u2010 and light\u2010chain mRNA of II Burkitt lymphoma (BL) cell lines (9 African and 2 American) were analyzed for various structural characteristics. In agreement with previous results at the protein level, all the BL cell lines express heavy\u2010chain mRNA transcripts of the \u03bc class. Surprisingly, a high \u03bc\/\u03bc<jats:sub>m<\/jats:sub> mRNA ratio was found in 2 lgM\u2010producing BL cell lines (Raji and CCL85), that do not secrete immunoglobulin. Variable region gene use was also assessed in the cell lines: while 4 out of 7 endemic BL cell lines use V<jats:sub>H<\/jats:sub> genes that belong to the V<jats:sub>H<\/jats:sub><jats:sup>3<\/jats:sup> gene family, no clear bias in the expression of particular V<jats:sub>H<\/jats:sub> or V<jats:sub>L<\/jats:sub> gene families among this sampling of BL lines was found. Northern blot analysis of immunoglobulin transcripts in endemic BL cell lines did show that 2 such lines (AG876 and HTB62) expressed truncated heavy\u2010chain transcripts; RNA sequence analysis of the V<jats:sub>H<\/jats:sub> region demonstrated different abnormal 5\u2010localized RNA splicing events for the 2 shortened \u03bc transcripts. The light\u2010chain mRNA in these 2 cell lines also showed structural abnormalities and, in the case of HTB62, 3 different K light\u2010chain transcripts are produced (of elongated, native and truncated sizes). <jats:italic>In vitro<\/jats:italic> translation of mRNA from HTB62 showed \u03bc and K chain proteins corresponding with the relative size for each message.<\/jats:p>","DOI":"10.1002\/ijc.2910430534","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:45:49Z","timestamp":1171921549000},"page":"930-935","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of immunoglobulin mrna expression in burkitt lymphoma cell lines"],"prefix":"10.1002","volume":"43","author":[{"given":"Roberto","family":"Anker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Caldwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Brokaw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian A.","family":"Pollok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113722"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.10.3260"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90243-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.8.2689"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.4049\/jimmunol.129.3.1336","article-title":"Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non\u2010Burkitt's type","volume":"129","author":"Benjamin D.","year":"1982","journal-title":"J. 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