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The number of insulin\u2010binding sites on intact Burkitt cells was decreased by &gt;90% compared to lymphoblastoid cells, with no change in affinity. This decrease in binding was paralleled by reduced amounts of insulin receptor \u03b1 (M<jats:sub>r<\/jats:sub> 130,000) and \u03b2 (M<jats:sub>r<\/jats:sub> 95,000) subunits detected by cell\u2010surface\u2010labelling and insulin receptor mRNA transcripts, indicating that transcription of receptor mRNA is decreased in Burkitt cells compared to lymphoblastoid cells and\/or that receptor mRNA is less stable. Burkitt cells displayed negligible insulin\u2010stimulated \u03b2 subunit auto\u2010phosphorylation, which could reflect either their decreased number of receptors or a defect in signal transduction. Structural analysis also revealed that the Burkitt cells had an increase in a precursor form (M<jats:sub>r<\/jats:sub>210,000) of the receptor, suggesting that decreased expression of the receptor may be associated with defective processing. Four Burkitt cell lines with t(8;l4) also had reductions of 45\u2013100% in expression of class\u2010l major histocompatibility (MHC) antigens. The expression of insulin receptors in both Burkitt and lymphoblastoid cells correlated with the expression of class\u2010l MHC antigens. There was also an inverse correlation between the expression of c\u2010myc and both insulin receptors and class\u2010l MHC antigens. As the insulin receptor is absent on resting B cells and is induced after cell activation, the decrease in receptor expression on Burkitt cells may reflect their less activated phenotype compared to lymphoblastoid cells.<\/jats:p>","DOI":"10.1002\/ijc.2910440315","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:40:03Z","timestamp":1171921203000},"page":"467-473","source":"Crossref","is-referenced-by-count":5,"title":["Insulin receptor expression in burkitt lymphoma cell lines"],"prefix":"10.1002","volume":"44","author":[{"given":"Julie D.","family":"Newman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard C.","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.7.1982"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01309995"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1983.tb01749.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90509-X"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90507-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6401867"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/274756a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.5.1135"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1007\/BF00265023","article-title":"Analysis of insulin receptors on heterogeneous eukaryotic cell populations with fluo\u2010rochrome\u2010conjugated insulin and fluorescence\u2010activated cell sorter. 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