{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:34:07Z","timestamp":1761762847470},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":6120,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1989,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monoclonal antibodies (MAbs) were obtained from hybrid\u2010oma clones established by cell fusion between P3X63Ag8.653 mouse myeloma cells and spleen cells of mice or rats hyper\u2010immunized against human bladder cancer tissue or BC47 rat bladder cancer cells. RBS\u201031 and RBS\u201085 mouse MAbs and RBA\u20101 rat MAb were raised against BC47 cells and HBP\u20101 MAb was raised against human bladder cancer tissues. Urinary antigens detected by these MAbs were quantitatively assayed by means of ELISA using 50 \u03bc1 of 1:2 diluted urine samples. The cut\u2010off value of the assay was set up as the mean + 4 \u00d7 SD of the mean using data from the healthy individual urine samples. The reactivity of all healthy control urine samples were under the cut\u2010off value (negative). By contrast, urine from bladder cancer patients reacted positively with the RBS\u201031 MAb at 72%, with the RBS\u201085 MAb at 63%, with the RBA\u20101 MAb at 51% and with the HBP\u20101 MAb at 35%. The urine samples from some patients with renal calculi, acute cystitis or complicated urinary tract infections showed only a weak reactivity with our MAbs. As for extra\u2010bladder cancers, some patients with renal, renal pelvis, prostate or ureter cancer, but no patients with esophageal, gastric, colon or liver cancer or leukemia, had reactive urinary antigens.<\/jats:p>","DOI":"10.1002\/ijc.2910440404","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:39:55Z","timestamp":1171921195000},"page":"582-588","source":"Crossref","is-referenced-by-count":4,"title":["Increase in murine monoclonal\u2010antibody\u2010defined urinary antigens in patients with bladder cancer and benign urogenital disease"],"prefix":"10.1002","volume":"44","author":[{"given":"Takashi","family":"Masuko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunio","family":"Sugahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Kamiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910350614"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.1.224"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.20.7227"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/80.15.1203"},{"key":"e_1_2_1_6_1","first-page":"329","article-title":"Hybridoma established from spleen cells of a rat immune to syngeneic bladder tumor: its secretion of monoclonal antibody specific for a tumor cell clone in a long\u2010term transplanted bladder tumor line","volume":"72","author":"Hashimoto Y.","year":"1981","journal-title":"Gann"},{"key":"e_1_2_1_7_1","first-page":"818","article-title":"A proliferation\u2010associated rat cell surface antigen recognized by a murine monoclonal antibody","volume":"74","author":"Hashimoto Y.","year":"1983","journal-title":"Gann"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90677-7"},{"key":"e_1_2_1_9_1","first-page":"2210","article-title":"Monoclonal antibodies to cell surface antigens shared by chemically induced mouse bladder carcinomas","volume":"45","author":"Hellstr\u00f6m I.","year":"1985","journal-title":"Cancer Res."},{"key":"e_1_2_1_10_1","first-page":"3868","article-title":"Urinary glycosaminoglycan excretion as a biochemical marker in patients with bladder cancer","volume":"41","author":"Hennessey P. 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