{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:11:36Z","timestamp":1758280296200},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":6120,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1989,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A human neuroblastoma cell line, CA\u20102E, has been established from a bone\u2010marrow aspirate of a 16\u2010month\u2010old boy with progressive disease. The karyotype and antigen pheno\u2010type of the cells correspond to those of a neuroblastoma. This cell line grows well in liquid cultures supplemented with 5% fetal calf serum; conversely, colony formation in semi\u2010solid medium by cells from early passages is dependent upon exogenous EGF. With time in continuous culture, the cloning efficiency in the absence of EGF increases, but the line remains sensitive to EGF, as evidenced by an enhancement of the number and size of colonies. A relative dependence upon EGF in liquid cultures has also been clearly demonstrated by limiting the concentration of serum. Long\u2010term (over 2 weeks) treatment with EGF results in a decreased rate of proliferation, a decreased proportion of clonogenic cells, and the appearance of flat, epithelial\u2010type cells. In some experiments, EGF also has a remarkable effect in inducing neurite outgrowth and process branching. Our results suggest that EGF may have both proliferation\u2010 and differentiation\u2010inducing effects on this neuroblastoma cell line. We have also shown that EGF induces increased proliferation in 7 out of 8 other human neuroblastoma cell lines. Functional response of neuroblastoma cells to EGF appears to be a general phenomenon which may be related to a block in the normal maturation pathway of the neural crest cells from which this tumor originates.<\/jats:p>","DOI":"10.1002\/ijc.2910440418","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:39:55Z","timestamp":1171921195000},"page":"661-668","source":"Crossref","is-referenced-by-count":13,"title":["Epidermal growth factor responsiveness of a new human neuroblastoma cell line"],"prefix":"10.1002","volume":"44","author":[{"given":"L.","family":"Suardet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Gross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.\u2010C.","family":"Gaide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Beck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"Eliason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(85)90214-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910390118"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1987.238"},{"key":"e_1_2_1_5_1","first-page":"2643","article-title":"Morphology and growth, tumorigencity. and cytogenetics of human neuroblastoma cells in continuous culture","volume":"33","author":"Biedler J. L.","year":"1973","journal-title":"Cancer Res."},{"key":"e_1_2_1_6_1","first-page":"4678","article-title":"Cytogenetic features of human neuroblastoma cell lines","volume":"41","author":"Brodeur G. M.","year":"1981","journal-title":"Cancer Res."},{"key":"e_1_2_1_7_1","unstructured":"B\u00fcrgisser P. Mise an point d'un dosage radio\u2010unmunologiqne de la Prot\u00e9ine basique de la my\u00e9litic dans le liquide c\u00e9phalo\u2010rachidien el dans le tissu nerveux. Application \u00e0 la clinitue el \u00e0 la recherche. Thesis University of Lausanne (1983)."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.48.070179.001205"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83739-0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)91606-0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1985.195"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90189-3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/10.3.348"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1150465"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0045-6039(86)90021-7"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320280409"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910390420"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910430421"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910360612"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90476-0"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(84)90477-0"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/313144a0"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240380203"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3498986"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240210108"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.4.1385"},{"key":"e_1_2_1_27_1","first-page":"4726","article-title":"Expression of epidermal growth factor receptor in human cultured cells and tissues: relationship to cell lineage and stage of differentiation","volume":"46","author":"Real F. X.","year":"1986","journal-title":"Cancer Res."},{"key":"e_1_2_1_28_1","first-page":"161","article-title":"Amplified and overexpressed epidermal growth factor receptor gene in uncultured primary human breast carcinoma","volume":"48","author":"Ro J.","year":"1988","journal-title":"Cancer Res."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)90287-X"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490080226"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198010093031511"},{"key":"e_1_2_1_32_1","first-page":"2531","article-title":"Schwannian cell differentiation of human neuroblastoma cell lines in vitro induced by bromodeoxyuridine","volume":"48","author":"Sugimoto T.","year":"1988","journal-title":"Cancer Res."},{"key":"e_1_2_1_33_1","first-page":"51","article-title":"Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia\u2010lymphoma cell lines by a panel of 38 monoclonal antibodies","volume":"75","author":"Sugimoto T.","year":"1984","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/309418a0"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3500513"},{"key":"e_1_2_1_36_1","first-page":"1372","article-title":"Adrenergic, cholinergic and inactive human neuroblastoma cell lines with the action\u2010potential Na+ ionophore","volume":"37","author":"West G. J.","year":"1977","journal-title":"Cancer Res."}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910440418","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910440418","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T16:46:43Z","timestamp":1697993203000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910440418"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,15]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,10,15]]}},"alternative-id":["10.1002\/ijc.2910440418"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910440418","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,15]]}}}