{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T10:45:57Z","timestamp":1698144357870},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":6028,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1990,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The phenotypic and molecular genetic characteristics of 4 variant sublines of the Burkitt lymphoma cell line Namalwa have been examined. The sublines are DNA\u2010fingerprint\u2010identical and derived from a monoclonal tumour, as shown by a rearrangement of the T\u2010cell\u2010receptor \u03b2\u2010chain gene common to the 4 sublines. There is non\u2010co\u2010ordinate expression of MHC class\u20101, MHC class\u2010II, surface immunoglobulin and a number of antigens recognized by CD MAbs on the different sublines. These different phenotypes of the cells are reminiscent of B cells arrested in varying states of cellular maturity. On Southern blots there are different patterns of restriction fragments hybridizing with Ig heavy\u2010 and light\u2010chain gene probes among the sublines, indicating that multiple rearrangements or mutations of 1g genes have occurred in the cells. Different patterns of hybridizing fragments among the sublines were also found by using c\u2010<jats:italic>myc<\/jats:italic> probes, implying the existence of different mutations of the c\u2010<jats:italic>myc<\/jats:italic> locus. The c\u2010<jats:italic>myc<\/jats:italic> mutation found in one of the sublines mapped to the 5\u2032 flanking sequence and in another 3\u2032 to the c\u2010<jats:italic>myc<\/jats:italic> locus. Using the J17BS8 probe, which detects a restriction fragment length polymorphism in the 3\u2032 flanking region of the c\u2010<jats:italic>myc<\/jats:italic> gene, a 4\u2010fold variation in the gene copy number among the sublines was found and one of the sublines was shown to be hemizygous for c\u2010<jats:italic>myc<\/jats:italic>. Examination of DNA from early cultures of Namalwa cells showed that the alterations in Ig and c\u2010<jats:italic>myc<\/jats:italic> structure had occurred on prolonged culture of the cells.<\/jats:p>","DOI":"10.1002\/ijc.2910450120","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:00:18Z","timestamp":1171922418000},"page":"109-118","source":"Crossref","is-referenced-by-count":9,"title":["Recurrent mutation of immunoglobulin and c\u2010<i>myc<\/i> genes and differential expression of cell surface antigens occur in variant cell lines derived from a burkitt lymphoma"],"prefix":"10.1002","volume":"45","author":[{"given":"K.","family":"Guy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. G.","family":"Middleton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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