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When injected subcutaneously in sublethally irradiated, splenectomized and anti\u2010asialo\u2010GM<jats:sub>1<\/jats:sub>\u2010treated nude mice, ZS cells invaded the lymph nodes, that appeared 10 to 50\u2010fold enlarged in all of the mice tested. Furthermore, ZS cells expressed a typical T\u2010cell surface structure, the CD2 molecule, detectable by a variety of different anti\u2010CD2 monoclonal antibodies (MAbs). However, other T\u2010cell markers were not found, with the possible exception of a truncated messenger of the \u03b2 chain of the T\u2010cell receptor and ZS cells could be identified as B cells since they (i) expressed a battery of markers of the resting and activated B cells, (ii) displayed a monoclonal rearrangement of the lgH chain locus and (iii) synthesized lgM K molecules.<\/jats:p><jats:p>The Epstein\u2010Barr virus (EBV) genome was detected in ZS cells in approximately ten copies per cell by DNA hybridization techniques. Furthermore, the cells were positive for EBV nuclear antigens (EBNA). Western blotting analysis of EBV encoded antigens demonstrated clear differences with those present in the B 95.8 virus\u2010producer cell line, indicating that ZS cells were not infected by EBV <jats:italic>in vitro<\/jats:italic> and that they already harbored the virus <jats:italic>in vivo<\/jats:italic>. ZS cells formed colonies <jats:italic>in vitro<\/jats:italic> with a high cloning efficiency and displayed chromosomal abnormalities in all of the mitoses (karyotype 47, xy, +13,\u201314, 8p+, 21p+, +m). In spite of these malignant features, ZS cells expressed the full range of EBV latent proteins as usually do \u201cnormal\u201d LCSs and did not have any of the chromosomal abnormalities that juxtapose the <jats:italic>c\u2010myc<\/jats:italic> oncogene to one of the genes coding for immunoglobulir molecules.<\/jats:p>","DOI":"10.1002\/ijc.2910450216","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:59:30Z","timestamp":1171922370000},"page":"299-307","source":"Crossref","is-referenced-by-count":11,"title":["Establishment of an EBV\u2010positive lymphoblastoid cell line that grows as a lymphoma in nude mice and expresses membrane CD2 molecules"],"prefix":"10.1002","volume":"45","author":[{"given":"S.","family":"Roncella","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dunia","family":"Ramarli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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