{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:24:35Z","timestamp":1793118275425,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":5908,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1990,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two cell lines (University of Michigan squamous carcinoma of the vulva UM\u2010SCV\u20101A and UM\u2010SCV\u20101B) were established from the primary tumor and a malignant pleural effusion of a 62\u2010year\u2010old woman. Both tumor specimens grew vigorously <jats:italic>in vitro<\/jats:italic> and could be passaged after only 14 and 10 days in culture, respectively. Both cell lines undergo 3 population doublings in 4 days, reaching saturation densities of 5 \u00d7 10<jats:sup>5<\/jats:sup> cells\/cm<jats:sup>2<\/jats:sup>, and have been carried through more than 30 <jats:italic>in vitro<\/jats:italic> passages. In nude mice the cultured cells initially formed tumors but these regressed 2\u20133 weeks after inoculation. The regressing mouse tumors consisted of poorly differentiated squamous carcinoma surrounded by an inflammatory lymphoid infiltrate. The UM\u2010SCV\u20101 cell lines express membrane antigens typically displayed by squamous\u2010cell carcinomas. These include the HLA class\u20101 light chain \u03b2<jats:sub>2<\/jats:sub>\u2010microglobulin, pemphigus, pemphigoid, and the \u03b1<jats:sup>6<\/jats:sup>\u03b2<jats:sub>4<\/jats:sub> integrin defined by the UM\u2010A9 monoclonal antibody (MAb). In contrast to the A431 vulvar carcinoma, these tumor lines do not have amplified expression of the epidermal growth factor (EGF) receptor. Although tissue from the primary tumor contained low levels of estrogen receptor activity, no receptor activity was detected in the cell lines. Nevertheless, both lines were sensitive to growth inhibition by tamoxifen. This effect was not reversible by estradiol, indicating an estrogen\u2010receptor\u2010independent mechanism. The tumors were both hypotetraploid, contained the same chromosome rearrangements and had stable karyotypes <jats:italic>in vitro<\/jats:italic>. Each contained inv(1)(p36.3q32.1), del(4)(q12), dic(4;11)(q12;p11.2), i(5p), der(6)t(3;6)(q25.1;p21.1), several rearrangements involving chromosomes 8 and 14, +i(13), i(18p), a dicentric t(11;19), and 2 or 3 unidentified markers. Since the karyotypes of both tumors were the same, no major karyotypic change was associated with metastatic spread. These paired primary and metastatic SCC lines from an unusually aggressive vulvar carcinoma provide an <jats:italic>in vitro<\/jats:italic> model for analysis of the biological basis of this tumor's behavior.<\/jats:p>","DOI":"10.1002\/ijc.2910450524","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:55:30Z","timestamp":1171922130000},"page":"920-927","source":"Crossref","is-referenced-by-count":25,"title":["Phenotypic characterization, karyotype analysis and <i>in vitro<\/i> tamoxifen sensitivity of new ER\u2010negative vulvar carcinoma cell lines, UM\u2010SCV\u20101A and UM\u2010SCV\u20101B"],"prefix":"10.1002","volume":"45","author":[{"given":"Seija E.","family":"Gr\u00e9nman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel L.","family":"Van Dyke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Worsham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"England","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth D.","family":"McClatchey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hopkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. Ramesh","family":"Babu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reidar","family":"Gr\u00e9nman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Carey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1259\/0007-1285-59-698-171"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/328614a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459988309100503"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459988709600301"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/305779a0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2294591"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000299017"},{"key":"e_1_2_1_9_1","first-page":"987","article-title":"Estrogen and progesterone receptor assays on the vulvar epithelium","volume":"31","author":"Garan J. 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