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The enzyme extracted from tumor\u2010digested collagen type I reconstituted fibrils and yielded the three\u2010quarter segments characteristic for the action of one of the matrix metalloproteinases: MMP\u2010I or mammalian collagenase. Metal\u2010chelating agents such as EDTA and O\u2010phenanthrolin indeed inhibited this activity. Collagenolytic activities were calculated on the basis of wet weight, total DNA and total extracted protein. Correlations were sought between levels of activity and both clinicopathological stage (Dukes' staging) and grade of histological differentiation. In all the assays applied, significant correlations were found between grade of histological differentiation and collagenolytic activity expressed as the tumor\/control ratios: poorly\u2010differentiated tumors exhibited a higher tumor\/control ratio than well\u2010differentiated tumors. Also, tumors penetrating into the serosa showed a higher tumor\/control ratio than tumors invading the <jats:italic>muscularis propria<\/jats:italic> only. A relation between collagenolytic activity and clinico\u2010pathological stage was observed only if activities were calculated on a DNA basis. These results confirm a relationship between the histological appearance of a tumor and its enzymatic potential to degrade interstitial collagens.<\/jats:p>","DOI":"10.1002\/ijc.2910450616","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T21:54:30Z","timestamp":1171922070000},"page":"1071-1078","source":"Crossref","is-referenced-by-count":54,"title":["Correlation between collagenolytic activity and grade of histological differentiation in colorectal tumors"],"prefix":"10.1002","volume":"45","author":[{"given":"Jos W. J. 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