{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T17:19:24Z","timestamp":1704993564657},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5675,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1991,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Somatic cell hybrids between the malignant Burkitt lymphoma cell line BL 60 and the non\u2010malignant Epstein\u2010Barr virus (EBV) immortalized lymphoblastoid cell line (LCL) IARC 277 demonstrate the deregulated c<jats:italic>\u2010myc<\/jats:italic> transcription pattern of the parental BL cell line during exponential growth in tissue culture. Subcutaneous nude mouse grafts of these hybrids, however, completely regress after an initial growth phase. To investigate whether regression of these grafts is mediated by a down\u2010regulation of the BL\u201060\u2010derived deregulated c<jats:italic>\u2010myc<\/jats:italic> gene in vivo, c<jats:italic>\u2010myc<\/jats:italic> transcription was analyzed in growing versus regressing hybrid grafts. In the initial growth phase as well as during regression, these grafts showed the deregulated c<jats:italic>\u2010myc<\/jats:italic> expression pattern of the parental BL cell line with highly abundant c<jats:italic>\u2010myc<\/jats:italic> transcripts originating from the BL specific translocation chromosome 8q<jats:sup>+<\/jats:sup>. Our results question the significance of c<jats:italic>\u2010myc<\/jats:italic> deregulation for an unlimited in vivo growth potential of B\u2010lymphoblastoid cells. To further characterize the hybrids, surface expression of B\u2010cell\u2010specific antigens was analyzed on the hybrids and shown to correspond to that of the parental LCL. In addition, transcription of the gene encoding for the EBV latent membrane protein (LMP) as well as histological features were analyzed in growing versus regressing hybrid grafts. The LMP gene, which is down\u2010regulated in the tumors produced by the parental BL cells, was expressed in the hybrid grafts as well as in the parental LCL grafts. This finding might be compatible with a host response of the nude mouse against LMP. The histological analysis, however, which revealed massive necrosis in the center of the regressing grafts without pronounced inflammatory infiltrates, rather points to a hypoxemic process leading to graft regression.<\/jats:p>","DOI":"10.1002\/ijc.2910470118","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:08:06Z","timestamp":1171922886000},"page":"99-104","source":"Crossref","is-referenced-by-count":8,"title":["Regressing nude mouse grafts of burkitt's lymphoma\u00d7lymphoblastoid cell hybrids show deregulation of the c\u2010myc gene and expression of the EBV latent membrane protein"],"prefix":"10.1002","volume":"47","author":[{"given":"J\u00fcrgen","family":"Wolf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Britta","family":"Klevenz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Pawlita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrij","family":"Komitowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerd","family":"Moldenhauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald Zur","family":"Hausen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","unstructured":"Bosch F. Schwarz E. Boukamp P. Fusenig N. E. Bartsch D.andzur Hausen H. Suppressionin vivoof human papillomavirus type 18 E6\u2010E7 gene expression in non\u2010tumorigenic HeLa\u2010fibroblast hybrid cells.J. Virol.(In press) (1990).","DOI":"10.1128\/jvi.64.10.4743-4754.1990"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711480405"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3685977"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910380517"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910410117"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1988.7.297"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910450332"},{"key":"e_1_2_1_10_1","volume-title":"Lymphocyte typing IV. White cell differentiation antigens","author":"Knapp W.","year":"1989"},{"key":"e_1_2_1_11_1","series-title":"IARC Scientific Publication 60","first-page":"309","volume-title":"Burkitt's lymphoma: a human cancer model","author":"Lenoir G. 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