{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T05:40:28Z","timestamp":1698126028277},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":5646,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1991,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A heteroconjugate (HC) was synthesized between OKT3 and monoclonal antibody (MAb) 7E8, which specifically reacts with the tumor marker placental alkaline phosphatase (PLAP). Similarly to OKT3, <jats:italic>in vitro<\/jats:italic>, the HC induced a dose\u2010dependent proliferation response of human peripheral\u2010blood mononuclear cells (PBMCs) and, in concert with rlL\u20102, it progressively activated T cells over a 4\u2010day period. In co\u2010cultures of continuously activated PBMCs and M0<jats:sub>4<\/jats:sub> tumor cells (non\u2010MHC\u2010restricted mouse fibroblasts transfected with the cDNA for PLAP), the HC (25 ng\/ml), again acting in concert with rlL\u20102, induced specific lysis of the MO<jats:sub>4<\/jats:sub> cells. This process occurred progressively over 2 to 3 days and was monitored from the release in the supernatant fluid of cellular <jats:sup>3<\/jats:sup>H\u2010L\u2010leucine, but also from analyses involving the remaining non\u2010lysed cancer cells, <jats:italic>i.e.<\/jats:italic>, by estimates of their protein content, by measurements of their viability, and most accurately by determinations of their PLAP content. Antibody 7E8 by itself induced a weak tumor\u2010cell lysis (ADCC), potentiated by the addition of rlL\u20102. However, after 7 days of PBMC\u2010preactivation with the HC and rlL\u20102, antibody 7E8 no longer mediated any ADCC, whereas the HC\u2010dependent lysis was further potentiated. The observed proliferation of T cells and development of cytotoxicity at low concentrations of HC and rlL\u20102 support the idea that a moderate but continuous T\u2010cell activation combined with T\u2010cell targeting is sufficient for the induction of progressive and efficient tumor\u2010cell lysis.<\/jats:p>","DOI":"10.1002\/ijc.2910470321","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:05:43Z","timestamp":1171922743000},"page":"431-438","source":"Crossref","is-referenced-by-count":3,"title":["Tumor\u2010cell lysis by <i>in\u2010situ<\/i>\u2010activated human peripheral\u2010blood mononuclear cells"],"prefix":"10.1002","volume":"47","author":[{"given":"Karine A.","family":"Smans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc F.","family":"Hoylaerts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herman F.","family":"Hendrickx","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine J.","family":"Goergen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc E.","family":"De Broe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910430327"},{"key":"e_1_2_1_3_1","first-page":"29","article-title":"Cell\u2010cell interactions. Clin. Immunol","volume":"6","author":"Bolhuis R. L. H.","year":"1986","journal-title":"Allergy"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.4049\/jimmunol.138.6.1779","article-title":"Inter\u2010leukin\u20102\u2010activated human lymphocytes exhibit enhanced adhesion to normal vascular endothelial cells and cause their lysis","volume":"138","author":"Damle N. K.","year":"1987","journal-title":"J Immunol."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198803000-00009"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1093\/clinchem\/34.10.1995","article-title":"Multicenter evaluation of human placental alkaline phosphatase as a possible tumor\u2010associated antigen in serum","volume":"43","author":"De Broe M. E.","year":"1988","journal-title":"Clin. Chem."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)92495-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"2555","DOI":"10.4049\/jimmunol.125.6.2555","article-title":"Thymoma production of T\u2010cell growth factor (interleukin 2)","volume":"125","author":"Farrar J. J.","year":"1980","journal-title":"J. Immunol"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-108-4-518"},{"key":"e_1_2_1_10_1","first-page":"63","article-title":"Regan isoenzyme and human chorionic gonadotropin in ovarian cancer","volume":"42","author":"Fishman W. H.","year":"1975","journal-title":"Nat. Cancer Inst. Monogr."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(82)90046-8"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.155.6.1823"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1093\/clinchem\/36.10.1793","article-title":"Accurate detection of human placental and germ\u2010cell alkaline phosphatase in serum","volume":"36","author":"Hendrix P. G.","year":"1990","journal-title":"Clin. Chem."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.17.6342"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910340604"},{"key":"e_1_2_1_16_1","first-page":"923","article-title":"Characterization of spontaneous, chemical and viral transformants of a C3H\/3T3\u2010type mouse cell line by transplantation into young chick blastoderms","volume":"54","author":"Mareel M.","year":"1975","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(84)90122-0"},{"key":"e_1_2_1_18_1","first-page":"51","article-title":"Influence of implantation site of MO4 cell aggregates on the formation of metastasis","volume":"2","author":"Meyvisch C.","year":"1982","journal-title":"Invasion Metastasis"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(75)90398-8"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6332379"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90205-J"},{"key":"e_1_2_1_23_1","first-page":"230","article-title":"Tumor markers in the human ovary and its neoplasms: a comparative immunohistochemical study","volume":"126","author":"Nouwen E. J.","year":"1987","journal-title":"Amer. J. Pathol."},{"key":"e_1_2_1_24_1","first-page":"866","article-title":"Immunohistochemical localization of placental alkaline phosphatase, carcinoembryonic antigen and cancer antigen 125 in the normal and neoplastic human lung","volume":"46","author":"Nouwen E. J.","year":"1986","journal-title":"Cancer Res."},{"key":"e_1_2_1_25_1","first-page":"377","article-title":"The DATECA Study group. Evaluation of a new, sensitive radioimmunoassay for placental alkaline phosphatase in pre\u2010 and post\u2010operative sera from the Danish testicular cancer material","volume":"166","author":"Nustad K.","year":"1984","journal-title":"Progress clin. biol. Res."},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.164.4.1193"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.4049\/jimmunol.137.7.2069","article-title":"Specific lysis of human tumor cells by T cells coated with anti\u2010T3 cross\u2010linked to antitumor antibody","volume":"137","author":"Perez P.","year":"1986","journal-title":"J. Immunol."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.5.1169"},{"key":"e_1_2_1_29_1","first-page":"731","article-title":"IFCC methods for the measurement of catalytic concentrations of enzymes. Part 5: IFCC method for alkaline phosphatases","volume":"21","author":"Tietz N. W.","year":"1983","journal-title":"J. clin. chem. clin. Biochem."},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"4018","DOI":"10.4049\/jimmunol.138.11.4018","article-title":"Human T cells targeted with anti\u2010T3 crosslinked to anti\u2010tumor antibody prevent tumor growth in nude mice","volume":"138","author":"Titus J. A.","year":"1987","journal-title":"J. Immunol"},{"key":"e_1_2_1_31_1","first-page":"1834","article-title":"Effects of ricin A\u2010chain conjugates of monoclonal antibodies to human alpha\u2010fetoprotein and placental alkaline phosphatase on antigen\u2010producing tumor cells in culture","volume":"45","author":"Tsukazaki K.","year":"1985","journal-title":"Cancer Res."},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910430230"},{"key":"e_1_2_1_33_1","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.4049\/jimmunol.124.6.2708","article-title":"OKT3: a monoclonal anti\u2010human T\u2010lymphocyte antibody with potent mitogenic properties","volume":"124","author":"van Wauwe J. P.","year":"1980","journal-title":"J. Immunol."}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910470321","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910470321","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T04:33:20Z","timestamp":1698035600000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910470321"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,2]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,2]]}},"alternative-id":["10.1002\/ijc.2910470321"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910470321","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,2]]}}}