{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T18:29:46Z","timestamp":1775586586636,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5507,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1991,6,19]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gp350, a late Epstein\u2010Barr\u2010virus (EBV) glycoprotein expressed on both the envelope of viral particles and EBV\u2010producing cells, is also the candidate for the development of an anti\u2010EBV subunit vaccine. This glycoprotein is thought to play an important role in anti\u2010EBV immunity. However, studies on the role of this viral antigen in cellular cytotoxicity and other immune functions have been hampered by the lack of a suitable model expressing gp350. We describe here a study in which we successfully transfected a gp350\u2010negative cell line resistant to natural\u2010killer(NK)\u2010cell activity (<jats:italic>i.e.<\/jats:italic>, Raji) with a recombinant plasmid (pZIP\u2010MA) containing the EBV\u2010gp350 and the neomycin resistance gene. Three clones with a stable and strong expression of gp350 on their surface membrane, as demonstrated using a gp350\u2010specific (<jats:italic>i.e.<\/jats:italic>, 2L10) monoclonal antibody (MAb) were isolated, characterized and used as targets in an antibody\u2010dependent cellular cytotoxicity (ADCC) assay. However, gp350 expression on 2 of the 3 isolated clones was not recognized by an anti\u2010gp350 MAb (72AI) which is specific to a unique gp350 epitope with a dual function (<jats:italic>i.e.<\/jats:italic>, involved in both EBV binding to its target cell receptors and in Inducing virus\u2010neutralizing antibody). We have also found that gp350 expression on our 3 selected clones does not affect EBV\u2010receptor (CR2) density. Our model of gp350\u2010expressing, NK\u2010cell\u2010activity\u2010resistant targets revealed very useful In determining that gp350 serves as a target antigen for EBV\u2010specific ADCC. These gp350\u2010expressing cell clones appear to represent a valuable tool for diagnostic purposes (<jats:italic>i.e.<\/jats:italic>, for detecting and titrating gp350 antibodies in patients with EBV\u2010associated diseases). Our approach should also prove useful for studying the expression of other cell\u2010surface\u2010associated viral and tumor antigens and their role in specific cellular immunity and immunosurveillance.<\/jats:p>","DOI":"10.1002\/ijc.2910480418","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:23:04Z","timestamp":1171923784000},"page":"591-597","source":"Crossref","is-referenced-by-count":2,"title":["Establishment and characterization of Epstein\u2010Barr virus gp350\u2010expressing transfected human lymphoid (Raji) cell clones"],"prefix":"10.1002","volume":"48","author":[{"given":"Menem","family":"Khyatti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pravin C.","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Menezes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1128\/jvi.54.3.665-674.1985","article-title":"Two major outer envelope glycoproteins of Epstein\u2010Barr virus are encoded by the same gene","volume":"54","author":"Beisel C.","year":"1985","journal-title":"J. Virol."},{"key":"e_1_2_1_3_1","first-page":"163","volume-title":"The Epstein\u2010Barr virus: recent advances","author":"Clearly M. L.","year":"1986"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90322-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-67236-1_14"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.59.1.94"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.157.3792.1064"},{"key":"e_1_2_1_8_1","first-page":"225","article-title":"Antibodies to Epstein\u2010Barr virus in nasopharyngeal carcinoma, other head and neck neoplasms, and control groups","volume":"44","author":"Henle W.","year":"1970","journal-title":"J. nat. 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