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Among the EBV transactivators, the ZEBRA protein plays a crucial role in switching the virus from a latent to a productive mode. ZEBRA protein was produced using a eukaryotlc expression vector: the open reading frame containing the BZFLI cDNA has previously been inserted downstream from the adenovirus major late promoter leading to expression of a 38\u2010kDa nuclear protein. We performed serological studies by employing ZEBRA protein expressed in human cells for immunofluorescence and Western\u2010blot assays. We were able to detect IgG anti\u2010ZEBRA antibodies (IgG\/ZEBRA) in 87% of NPC patients. These antibodies were absent in control sera; IgG\/ZEBRA antibodies can be proposed as a useful marker for diagnosis of NPC tumors.<\/jats:p>","DOI":"10.1002\/ijc.2910480503","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:22:45Z","timestamp":1171923765000},"page":"647-649","source":"Crossref","is-referenced-by-count":62,"title":["Detection of anti\u2010epstein\u2010barr\u2010virus transactivator (ZEBRA) antibodies in sera from patients with nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"48","author":[{"given":"Ir\u00e8ne","family":"Joab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Claude","family":"Nicolas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Schwaab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"de\u2010Th\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Clausse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Perricaudet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.10.6162"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04635.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"3672","DOI":"10.1128\/jvi.61.12.3672-3679.1987","article-title":"Polymorphic proteins encoded within BZLF1 of defective and standard Epstein\u2010Barr viruses disrupt latency","volume":"61","author":"Countryman J.","year":"1987","journal-title":"Virology"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.12.4085"},{"key":"e_1_2_1_6_1","first-page":"25","volume-title":"The herpesviruses","author":"De\u2010Th\u00e9 G.","year":"1983"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910430111"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(73)90341-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.1128\/jvi.62.7.2274-2284.1988","article-title":"A new Epstein\u2010Barr virus transactivator, R., induces expression of a cytoplasmic early antigen","volume":"62","author":"Hardwick J. 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