{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:51:27Z","timestamp":1790520687623,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5466,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1991,7,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cholanglocarcinoma (CCA) is one of the most common cancers In north\u2010east Thailand and has been associated with infestation by the liver fluke <jats:italic>Opisthorchis viverrini<\/jats:italic> (OV). Two samples of 12\u2010hr overnight urine (after dosing with proline and ascorbic acid or proline alone) were collected from 20 Inhabitants from each of 5 contrasting incidence areas for CCA. The incidence of CCA was not correlated with either the amount of NPRO or other nitrosamino acids, endogenous nitrosation potential (difference in NPRO levels between proline dose and proline and ascorbic dose), or nitrate level. However, when urinary levels of nitrosamino acids were compared in subjects living in high\u2010risk areas, subjects who were positive for OV antibody excreted significantly more (<jats:italic>P<\/jats:italic> &lt; 0.01) NPRO (12.3 \u00b1 18.7 \u03bcg\/12 hr) after proline ingestion than those who were negative (3.5 \u00b1 3.2 \u03bcg\/12 hr). After ingestion of ascorbic acid, the NPRO levels in the positive subjects were significantly reduced (<jats:italic>p<\/jats:italic> &lt; 0.01) to 2.4 \u00b1 2.0 \u03bcg\/12 hr, suggesting that endogenous nitrosation of proline was inhibited. Thus, endogenous nitrosation potential estimated from the difference between NPRO and the sum of nitrosamino acids excreted in the 2 urine samples was significantly higher in subjects positive for the OV antibody. Small amounts of pre\u2010formed nitrosamines were found in fermented fish and pork food items, which are consumed frequently in the high\u2010risk area for CCA. These results suggest that the interaction between chemical carcinogens, especially nitrosamines, and OV infestation may play a role in the development of cholangiocarcinoma in Thailand.<\/jats:p>","DOI":"10.1002\/ijc.2910480606","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:20:32Z","timestamp":1171923632000},"page":"821-825","source":"Crossref","is-referenced-by-count":75,"title":["<i>opisthorchis viverrini<\/i> infestation and endogenous nitrosamines as risk factors for cholangiocarcinoma in thailand"],"prefix":"10.1002","volume":"48","author":[{"given":"P.","family":"Srivatanakul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sukaryodhin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Ohshima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Khlat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Parkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Brouet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Bartsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"Statistical methods in medical research","author":"Armitage P.","year":"1987"},{"key":"e_1_2_1_3_1","unstructured":"Bartsch H. 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Parkin D. M. Jiang Y\u2010Z. Khlat M. Kao\u2010Ian U. Sontipong S.andWild C. The role of infection byOpisthorchis viverrini. Hepatitis B virus and aflatoxin exposure in the etiology of liver cancer in Thailand: a correlation study.Cancer(1991). (In press).","DOI":"10.1002\/1097-0142(19911201)68:11<2411::AID-CNCR2820681114>3.0.CO;2-0"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.1988.tb01394.x"},{"key":"e_1_2_1_25_1","first-page":"234","article-title":"Enzyme\u2010linked immunosorbent assay for detection of Opisthorchis viverrini infection","volume":"16","author":"Srivatanakul P.","year":"1985","journal-title":"Southeast Asian J. trop. Med. publ Hlth"},{"key":"e_1_2_1_26_1","first-page":"59","article-title":"An effective method of counting hookworm eggs in feces","volume":"3","author":"Stoll N. R.","year":"1923","journal-title":"Amer. J. 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