{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:45:55Z","timestamp":1757706355974},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5425,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1991,9,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Progestins have biological effects of regression and differentiation on human endometrial adenocarcinoma. We investigated the effects of progestin on the induction of macrophage\u2010colony\u2010stimulating factor (M\u2010CSF) and its receptor messenger RNAs in the human endometrial adenocarcinoma cell line Ishikawa which has receptors for both estrogen and progesterone. Poly(A)<jats:sup>+<\/jats:sup>RNA extracted from lshikawa cells cultured with or without synthetic progestin R5020 was subjected to Northern blot hybridization using M\u2010CSF and c\u2010<jats:italic>fms<\/jats:italic> cDNA probes. The expression of M\u2010CSF mRNA in Ishikawa cells increased about 2.3 times following treatment with R5020 at 10<jats:sup>\u22127<\/jats:sup> M. Induction of M\u2010CSF mRNA by RS020 was antagonized by anti\u2010progestin RU486 in a dose\u2010dependent manner. However, c\u2010<jats:italic>fms<\/jats:italic> mRNA, coding the M\u2010CSF receptor, was expressed constitutively in Ishikawa cells and its expression was not affected by hormonal treatment. We further examined the biological effects of M\u2010CSF on endometrial cancer cells. Colony formation of Ishikawa cells in soft agar, which represents anchorage\u2010independent cell growth, was inhibited by M\u2010CSF treatment. On the other hand, accumulation of glycogen granules in cytoplasm detected by periodicacid\u2010Schiff staining was observed in lshikawa cells treated with M\u2010CSF. These results indicate that M\u2010CSF, whose gene expression was enhanced by progestin, suppressed growth and induced differentiation of endometrial adenocarcinoma cells. These effects of M\u2010CSF on endometrial cancer cells are similar to those of progestins, so the effects of progestins on these cells are, at least in part, probably mediated by M\u2010CSF in an autocrine or paracrine manner.<\/jats:p>","DOI":"10.1002\/ijc.2910490215","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:19:14Z","timestamp":1171923554000},"page":"229-233","source":"Crossref","is-referenced-by-count":6,"title":["The biological effects of macrophage\u2010colony\u2010stimulating factor induced by progestin on growth and differentiation of endometrial adenocarcinoma cells"],"prefix":"10.1002","volume":"49","author":[{"given":"Tadashi","family":"Kimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chihiro","family":"Azuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumitaka","family":"Saji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihio","family":"Tokugawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiko","family":"Takemura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariko","family":"Miki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaaki","family":"Ono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Tanizawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"727","volume-title":"Moleculur biology of the cell","author":"Alberts B.","year":"1989"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8818"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0050103"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.164.3.956"},{"key":"e_1_2_1_6_1","first-page":"359","volume-title":"Molecular and cellular biology","author":"Dexter T. 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