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Five clones preferentially hybridized with pancreatic adenocarcinoma mRNA. cDNA inserts from 4 of these clones were amplified by PCR, labelled with \u03b1<jats:sup>32<\/jats:sup>P and used in Northern blot analysis against mRNA prepared from a variety of tumour and normal tissues. \u03bbGER\u20104 identified a pancreas\u2010associated mRNA (&gt; 10 kb) with no homology with known sequences at either the nucleic or amino\u2010acid level. \u03bbGER\u20102 identified a 1.7\u2010kb mRNA transcript that was over\u2010expressed in mRNA prepared from pancreas, colon, breast, lung and gastric tumours relative to normal tissues. Sequence analysis and restriction\u2010enzyme mapping showed that this clone was completely homologous with the active form of human elongation factor EF\u20101\u03b1. This high level of EF\u20101\u03b1\u2010mRNA expression in tumour tissues lends support to the increasing evidence that EF\u20101\u03b1 is an important regulator of the cell cycle.<\/jats:p>","DOI":"10.1002\/ijc.2910500513","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:39:08Z","timestamp":1171924748000},"page":"740-745","source":"Crossref","is-referenced-by-count":47,"title":["Differential screening of a human pancreatic adenocarcinoma \u03bbgt11 expression library has identified increased transcription of elongation factor ef\u20101\u03b1 in tumour cells"],"prefix":"10.1002","volume":"50","author":[{"given":"Anne G.","family":"Grant","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel M.","family":"Flomen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark L. 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