{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T22:31:45Z","timestamp":1717453905001},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":5200,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,4,22]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A model for testing chemotherapeutic agents in <jats:italic>vitro<\/jats:italic> is described. It is based on an organoid culture method which allows human carcinomas to grow <jats:italic>in vitro<\/jats:italic> and to maintain many typical <jats:italic>in vivo<\/jats:italic> properties, including 3\u2010dimensional architecture, growth of multiple cell types, expression of morphological differentiation and formation of histotypical structures. The preservation of drug sensitivity and resistance under the conditions of our organoid culture assay (OCA) was demonstrated by investigating 3 strains of a human hypopharynx carcinoma which differed by different sensitivity to cisplatin in <jats:italic>in vivo<\/jats:italic> conditions. These differences were retained <jats:italic>in vitro<\/jats:italic> and the modified neutral\u2010red (NR) assay was especially suitable for revealing drug\u2010induced cytotoxic damage in OCA. On the basis of our findings, the following approach is proposed for the <jats:italic>in vitro<\/jats:italic> testing of cytostatic drugs before they are administered to patients. (1) Removal of carcinomas from patients; (2) dense cell suspensions of these carcinomas to be dropped on membrane filters at the air\u2010medium interface, resulting in growth of solid nodules of organized carcinoma tissues; (3) addition of cytostatic drugs to the growth medium for 2 or 3 days; (4) detachment and bisection of the culture nodules; (5) determination of viable cells by NR uptake and total cell mass by the sulforhodamin B (SRB) assay; (6) determination of quotient NR:SRB absorbance, related as percentage to control value: this indicates the fraction of viable cells and gives a measure of the cytotoxic injury caused by the applied cytotoxic drug. Thus, the OCA seems to be suitable for defining the patterns of drug sensitivity and resistance of individual human carcinomas <jats:italic>in vitro<\/jats:italic> within a few days.<\/jats:p>","DOI":"10.1002\/ijc.2910510119","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:38:56Z","timestamp":1171924736000},"page":"99-107","source":"Crossref","is-referenced-by-count":11,"title":["An organoid culture assay (OCA) for determining the drug sensitivity of human tumors"],"prefix":"10.1002","volume":"51","author":[{"given":"P.","family":"K\u00f6pf\u2010Maier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Kolon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(85)90046-3"},{"key":"e_1_2_1_3_1","first-page":"421","volume-title":"Head and neck cancer","author":"Braakhuis B. 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