{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T07:58:38Z","timestamp":1770623918654,"version":"3.49.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":5200,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,4,22]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Deregulated c\u2010<jats:italic>myc<\/jats:italic> expression, as a consequence of translocation of the c\u2010<jats:italic>myc<\/jats:italic> gene to one of the immunoglobulin loci, appears to play an important role in the pathogenesis of several B\u2010cell tumors, including Burkitt's lymphoma, mouse plasmacytoma and rat immunocytoma. This study investigated the expression of c\u2010<jats:italic>myc<\/jats:italic> and 2 other members of the <jats:italic>myc<\/jats:italic> gene family, L\u2010 and N\u2010<jats:italic>myc<\/jats:italic>, at the mRNA and protein level, and analyzed for possible rearrangements of these genes in the human counterpart to the mouse plasmacytoma\u2014multiple myeloma (MM). Nine well\u2010characterized MM cell lines were examined by using Northern\u2010 and Southern\u2010blot analysis and immunoprecipitation. The c\u2010<jats:italic>myc<\/jats:italic> gene was found to be highly expressed in most MM cell lines. The level of expression was comparable to that observed in the COLO 320 and HL\u201060 cell lines, carrying amplified c\u2010<jats:italic>myc<\/jats:italic> genes, and to that of B\u2010cell lines with a higher proliferative activity than the MM cell lines. In the U\u2010266 MM cell line, L\u2010<jats:italic>myc<\/jats:italic>, but no c\u2010<jats:italic>myc<\/jats:italic> mRNA or protein, was found. The L\u2010<jats:italic>myc<\/jats:italic> gene was expressed in both early\u2010 and late\u2010passage U\u2010266 cells, suggesting that the L\u2010<jats:italic>myc<\/jats:italic> expression was not the result of the <jats:italic>in vitro<\/jats:italic> cultivation. N\u2010<jats:italic>myc<\/jats:italic> was not expressed in any of the MM cell lines. No rearrangements of c\u2010<jats:italic>myc<\/jats:italic> or L\u2010<jats:italic>myc<\/jats:italic> genes were found. We thus conclude that (a) in contrast to the corresponding mouse and rat B\u2010cell tumors, c\u2010<jats:italic>myc<\/jats:italic> is not frequently rearranged in MM; (b) c\u2010<jats:italic>myc<\/jats:italic> is highly expressed in most MM lines; and (c) L\u2010<jats:italic>myc<\/jats:italic> but not c\u2010<jats:italic>myc<\/jats:italic> is expressed in the U\u2010266 MM cell line.<\/jats:p>","DOI":"10.1002\/ijc.2910510121","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:38:56Z","timestamp":1171924736000},"page":"116-123","source":"Crossref","is-referenced-by-count":17,"title":["Expression of <i>myc<\/i>\u2010family genes in established human multiple myeloma cell lines: L\u2010<i>myc<\/i> but not c\u2010<i>myc<\/i> gene expression in the U\u2010266 myeloma cell line"],"prefix":"10.1002","volume":"51","author":[{"given":"H.","family":"Jernberg\u2010Wiklund","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Pettersson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u2010G.","family":"Larsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Anton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nilsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.6.1707"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1980.tb06030.x"},{"key":"e_1_2_1_4_1","first-page":"1359","article-title":"Amplification of the c\u2010myc and the pvt\u2010like region in human multiple myeloma","volume":"5","author":"Bakkus M. 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