{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T10:46:54Z","timestamp":1698144414570},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5121,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,7,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Seven human ocular melanoma cell lines were established <jats:italic>in vitro<\/jats:italic> and 3 of these, GU\u20104, LLN\u201040 and its subline C17\u201011, were characterized. Mice were immunized with these ocular melanoma cell lines, and 2 hybridomas producing monoclonal IgG<jats:sub>1<\/jats:sub>antibodies (MAb) were produced. MAb 2\/10SN recognizes a 44\u2010kDa monomeric protein, whereas MAb 10\/12SN reacts with an 83\/65\u2010kDa heterodimeric protein. These melanoma\u2010associated antigens (MAA) are detected at high concentrations in the cytoplasm of ocular melanoma cells. However, cell\u2010surface labelling techniques suggest that these MAA are also associated with the cell\u2010surface membrane. These 2 ocular MAA are also expressed by several skin melanoma cell lines. Immunohistochemical studies have localized these antigens to ocular and skin melanomas, to sweat ducts and basal squamous cells in normal skin, with limited expression in several other normal tissues and some carcinomas. Biodistribution studies in nude mice with human ocular melanomas have demonstrated good localization of <jats:sup>125<\/jats:sup>I\u2010labeled MAb 2\/10SN at the tumor sites. Therefore, these 2 MAbs, 2\/10SN and 10\/12SN, recognize MAA which appear to be unique and may prove useful for imaging purposes. <jats:italic>\u00a9 1992 Wifey\u2010Liss, Inc.<\/jats:italic><\/jats:p>","DOI":"10.1002\/ijc.2910510510","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:56:10Z","timestamp":1171925770000},"page":"718-726","source":"Crossref","is-referenced-by-count":1,"title":["Two antigens detected on human ocular melanomas with the mouse monoclonal antibodies 2\/10sn and 10\/12SN"],"prefix":"10.1002","volume":"51","author":[{"given":"Nour","family":"Safi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphe R. 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