{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:25:25Z","timestamp":1758633925182},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":5122,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,7,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The secretion of insulin\u2010like growth\u2010factor\u2010binding proteins (IGFBPs) and expression of the genes encoding IGFBP\u2010I, IGFBP\u20102 and IGFBP\u20103 have been studied in a panel of cell lines derived from breast carcinomas, Wilms' tumour, neuroblastoma, retinoblastoma, colon carcinoma, liver adenocarcinoma, Burkitt's lymphoma and a non\u2010small\u2010cell lung carcinoma. All cell lines, with the exception of the Burkitt's lymphoma cell line, secreted IGFBPs, as detected by affinity labelling. A 34\u2010kDa BP was present in the conditioned media of all IGFBP\u2010secreting cell lines, whereas BPs ranging from 18 kDa to 53 kDa were variably secreted. All IGFBP\u2010secreting cell lines expressed the IGFBP\u20102 gene as determined by Northern blot analysis. The Wilms' tumour, the neuroblastoma and the retinoblastoma cell line expressed the IGFBP\u20102 gene only. All other cell lines, with the exception of the Burkitt's lymphoma, expressed the IGFBP\u20102 gene and, in addition, either the IGFBP\u2010I gene and\/or the IGFBP\u20103 gene. IGFBP\u2010I gene expression could be detected by reverse transcriptase polymerase chain reaction only. IGFBP\u20103 gene expression was detected by Northern blot analysis, but transcripts were less abundant than IGFBP\u20102 mRNAs. These findings indicate that the expression of multiple BP genes and the secretion of BPs may be a common property of tumour cells. <jats:italic>\u00a9 1992 Wifey\u2010Liss, Inc.<\/jats:italic><\/jats:p>","DOI":"10.1002\/ijc.2910510525","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:56:10Z","timestamp":1171925770000},"page":"818-821","source":"Crossref","is-referenced-by-count":16,"title":["Insulin\u2010like growth\u2010factor\u2010binding protein gene expression and protein production by human tumour cell lines"],"prefix":"10.1002","volume":"51","author":[{"given":"Julie G.","family":"Reeve","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lemuel B.","family":"Kirby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ad","family":"Brinkman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie A.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jurg","family":"Schwander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman M.","family":"Bleehen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-2235(89)90041-0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb08386.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-4-9-1320"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03087.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-4-12-2039"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041480214"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"12210","DOI":"10.1016\/S0021-9258(19)38332-2","article-title":"Discrete alterations of the insulin\u2010like growth factor I molecule which alter its affinity for insulin\u2010like growth\u2010factor\u2010binding proteins result in changes in bioactivity","volume":"265","author":"Clemmons. 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