{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:36:49Z","timestamp":1761237409419},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5100,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,7,30]]},"abstract":"Abstract<\/jats:title>In a previous study on several independently established Epstein\u2010Barr virus (EBV)\u2010converted sublines of the EBV\u2010negative Burkitt lymphoma (BL) line BL41, we found that expression of the virally encoded membrane protein LMP I was accompanied by reduced agarose clonability and tumorigenicity. In order to investigate whether LMPI can induce these phenotypic changes by itself, we have now studied the growth in suspension culture, the clonability in agarose and the tumorigenicity in immunosuppressed and SCID mice of 4 LMPItransfected sublines of BL4I that carry the gene under the control of the ZnSO4<\/jats:sub>\u2010inducible metallothionein promoter. Expression of LMPI at levels comparable to those detected in EBV\u2010transformed lymphoblastoid cell lines (LCL) correlated with impairment of growth in suspension and reduction of clonability and tumorigenicity. Only minor changes were observed in transfectants expressing low LMPI levels. Upregulation of LMPI by ZnSO4<\/jats:sub> treatment of the low LMPI clone MTLM5 was accompanied by a slowing down of proliferation, increased cell clumping and decreased clonability. The results suggest that expression of LMP I at levels which are compatible with immortalization of normal B\u2010cells antagonizes the ability of BL cells to grow in vitro<\/jats:italic> and in vivo, and illustrate a possible mechanism by which down\u2010regulation of this viral antigen may favor tumorigenicity in EBV\u2010carrying BLs. \u00a9 1992 Wiley\u2010Liss, Inc<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/ijc.2910510619","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:55:19Z","timestamp":1171925719000},"page":"949-955","source":"Crossref","is-referenced-by-count":24,"title":["Expression of the epstein\u2010barr virus (EBV)\u2010encoded membrane protein LMP1 impairs the In vitro<\/i> growth, clonability and tumorigenicity of an EBV\u2010negative burkitt lymphoma line"],"prefix":"10.1002","volume":"51","author":[{"given":"Laura","family":"Cuomo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torbj\u00f6rn","family":"Ramquist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pankaj","family":"Trivedi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fred","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria G.","family":"Masucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"461","article-title":"Transformation of BALB 3T3 cells by the BNLF\u20101 gene of Epstein\u2010Barr virus","volume":"2","author":"Baichwal V. 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