{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T21:09:11Z","timestamp":1770412151311,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":5081,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,8,19]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human hepatoblastoma\u2010derived cell line HB611 secretes hepatitis\u2010B surface antigen (HBsAg) and hepatitis\u2010B e antigen (HBeAg) into the medium. Hepatitis\u2010B\u2010virus(HBV) DNA integrated into the cellular genome was found to be hypermethylated. When the cells were treated with 5\u2010azacytidine for 3 days, the level of HBsAg in the medium increased, while the level of HBeAg remained constant. The level of alphafetoprotein (AFP) decreased with the 5\u2010azacytidine treatment. Southern blot analysis of DNA digested with Hpall or Mspl showed that 5\u2010azacytidine treatment resulted in hypomethylation of the integrated HBV DNA, suggesting that 5\u2010azacytidine increased HBsAg production in the cells through hypomethylation of the HBV genomic DNA. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910520124","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:52:11Z","timestamp":1171925531000},"page":"137-140","source":"Crossref","is-referenced-by-count":10,"title":["Enhancement of hepatitis\u2010B surface\u2010antigen expression by 5\u2010azacytidine in a hepatitis\u2010B\u2010virus\u2010transfected cell line"],"prefix":"10.1002","volume":"52","author":[{"given":"Eiji","family":"Miyoshi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichi","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Towata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideyuki","family":"Fusamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takenobu","family":"Kamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoyuki","family":"Taniguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1989.tb02308.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000224981"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.52.070183.000521"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.13.4601"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(83)80232-0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.9.2534"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1128\/jvi.64.2.931-935.1990","article-title":"Transcription of the S gene in transgenic mouse is associated with hypomethylation at specific sites and with DNase\u2010I sensitivity","volume":"64","author":"Pourcel C.","year":"1990","journal-title":"J. Virology"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60678-8"},{"key":"e_1_2_1_11_1","first-page":"4968","article-title":"Human T\u2010Lymphotropic\u2010Virus type I transcriptional regulation by methylation","volume":"50","author":"Saggioro D.","year":"1990","journal-title":"Cancer Res."},{"key":"e_1_2_1_12_1","volume-title":"Molecular cloning: a laboratory manual (second edition)","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.2.444"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90057-3"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/225948a0"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/8.11.2461"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6194563"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.7.2207"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910520124","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910520124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T01:45:42Z","timestamp":1695865542000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910520124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,8,19]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,8,19]]}},"alternative-id":["10.1002\/ijc.2910520124"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910520124","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,8,19]]}}}