{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:57:17Z","timestamp":1761058637543},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5038,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Explant cultures were started from human papillomavirus (HPV)\u2010infected genital lesions in order to isolate and propagate abnormally differentiating cells from squamous intraepithelial neoplasia. A medium with high calcium concentration was used to induce terminal differentiation of cells from surrounding normal epithelium. Two cell lines with extended life\u2010spans were established. The UT\u2010DEC\u2010I cell line was derived from an HPV\u201033\u2010positive mild vaginal dysplasia (VAIN I). In cultured UT\u2010DEC\u2010I cells, HPV 33 DNA was detected with Southern\u2010blot hybridization and the polymerase chain reaction (PCR) technique. The restriction pattern of HPV 33 changed during early passages and flow cytometric analysis detected a decrease in chromosomal DNA content. HPV 33 RNA from the E6\u2010E7 region could be amplified by PCR at late passage. UT\u2010DEC\u20102 cell line was derived from an HPV\u201016\u2010positive moderate vaginal dysplasia (VAIN II). HPV 16 DNA was also detected in cultured cells by the PCR technique. The senescence of normal keratinocytes and growth selection in favor of aneuploid cells was observed by flow cytometric analysis at subsequent passages. Karyotype analysis showed clonal chromosomal abnormalities in both cell lines. To date, UT\u2010DEC\u20101 cells have undergone 40 and UT\u2010DEC\u20102 cells 25 passages. This study shows that the isolation of HPV\u2010infected dysplastic cells can be achieved by culturing the cells in a medium with high calcium concentration. The cell lines presented provide the opportunity of evaluating the early stages of squamous\u2010cell carcinogenesis. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910520311","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:51:25Z","timestamp":1171925485000},"page":"391-398","source":"Crossref","is-referenced-by-count":12,"title":["Isolation of two keratinocyte cell lines derived from HPV\u2010positive dysplastic vaginal lesions"],"prefix":"10.1002","volume":"52","author":[{"given":"Sakari","family":"Hietanen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eeva","family":"Auvinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seija","family":"Gr\u00e9nman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taina","family":"Lakkala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antti","family":"Sajantila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pekka","family":"Klemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juhani","family":"M\u00e4enp\u00e4\u00e4","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19910701)68:1<195::AID-CNCR2820680135>3.0.CO;2-L"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990100115"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0890-8508(89)90009-1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/321246a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01944.x"},{"key":"e_1_2_1_7_1","first-page":"251","article-title":"Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA","volume":"1","author":"D\u00fcrst M.","year":"1987","journal-title":"Oncogene"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.12.3812"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2537532"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910450524"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-72-7-1569"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(90)90249-T"},{"key":"e_1_2_1_13_1","volume-title":"Report of the standing committee on human cytogenetic nomenclature","author":"INTERNATIONAL SYSTEM FOR HUMAN CYTOGENETIC NOMENCLATURE.","year":"1985"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00155542"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90247-X"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.19.7169"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004347-198812000-00002"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02626466"},{"key":"e_1_2_1_19_1","first-page":"503","article-title":"Intraepithelial and invasive neoplasia of the vulva in association with human papillomavirus infection","volume":"33","author":"Planner R. 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