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Furthermore, we evaluated the tumor responses after either i.p. injection or topical application of 5\u2010ALA followed by laser irradiation (632 nm, 150 mW\/cm<jats:sup>2<\/jats:sup>, 25 min) by measuring the treated tumor regression\/regrowth time and by light and electron microscopy. Strong fluorescence of ALA\u2010induced porphyrins was detected in the tumor, skin and liver tissues, while little fluorescence was seen in the adjacent muscle tissue. Moreover, the highest amounts of ALA\u2010induced porphyrins in the tumor and skin tissues were found 1 hr after i.p. injection, whereas the amounts of the porphyrins in both tissues increased with increasing time after topical application of ALA. The fluorescence of the porphyrins was localized in several components of the skin tissue (epidermis, hair follicles and their associated sebaceous glands). Furthermore, the fluorescence was diffusely distributed in the s.c. transplanted tumor tissue. Little could be observed under a confocal laser scan microscope (CLSM) in the muscle tissue. The uptake and biolocalization data correlate well with the results of PCT efficiency of the same tumor model with ALA\u2010induced porphyrins. Light and electron microscopy showed that the mitochondria of the tumor cells and of the endothelial cells and the basal lamina of vascular walls beneath the endothelium in the tumor tissue were initially extensively destroyed after PCT with ALA\u2010induced porphyrins. Thereafter, diffuse degeneration followed by local and\/or diffuse severe necrosis of the tumor cells was found. This may be due mainly to the initial damage to mitochondria in the cancerous and endothelial cells and also to the destruction of the vascular wall in the tumor tissue. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910520318","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:51:25Z","timestamp":1171925485000},"page":"433-443","source":"Crossref","is-referenced-by-count":111,"title":["Distribution and photosensitizing efficiency of porphyrins induced by application of exogenous 5\u2010aminolevulinic acid in mice bearing mammary carcinoma"],"prefix":"10.1002","volume":"52","author":[{"given":"Q.","family":"Peng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Moan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Warloe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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