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In order to set up a reliable and simple routine test to determine Ip deletion in neuroblastoma, we have used the polymerase chain reaction to genotype neuroblastoma DNA at 2 loci containing a variable number of tandem repeats and located on the distal part of the short arm of chromosome I. Agarose gel electrophoresis and ethidium bromide staining of the amplification products enable a simple determination of constitutional and tumor genotypes at these loci to be made. A total of 37 samples from 29 patients were studied with this technique. Loss of heterozygosity (LOH) could be identified in 8 cases. In each case the results obtained were in agreement with those achieved by the Southern procedure. This technique will be of particular interest in the pretherapeutic analysis of Ip deletions in small tumor samples obtained by fine\u2010needle aspirates of the primary tumor. It should also enable retrospective studies from paraffin\u2010embedded tumor fragments to be made and provide information for the analysis of tumor heterogeneity in neuroblastoma and in other tumors with Ip deletions. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910520408","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:50:29Z","timestamp":1171925429000},"page":"544-548","source":"Crossref","is-referenced-by-count":46,"title":["PCR assay for chromosome 1p deletion in small neuroblastoma samples"],"prefix":"10.1002","volume":"52","author":[{"given":"Marline","family":"Peter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Michon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Vielh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia","family":"Neuenschwander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elise","family":"Sonsino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Michel","family":"Zucker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Vergnaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Delattre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910310510"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1988.6.12.1874"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19860715)58:2 <541::AID-CNCR2820581322>3.0.CO;2-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1988.24"},{"key":"e_1_2_1_6_1","first-page":"99","article-title":"Chromosome 1 interphase\u2010Cytogenetics in 32 primary neuroblastomas of different clinical stages","volume":"3","author":"Christiansen H.","year":"1991","journal-title":"Advanc. 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