{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:52:34Z","timestamp":1761540754863},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4925,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1993,1,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Experiments were designed in an attempt to identify T\u2010and B\u2010cell epitopes expressed on the 17\u2010kDa early\u2010antigen\u2010restricted (EA\u2010R) polypeptide of the EBV\u2010induced early antigen complex. Using Berzofsky's algorithm, 3 hypothetical T\u2010cell epitopes on p 17 were synthesized and employed in EBV\u2010specific lymphoproliferative assays. Lymphocytes from all EBV\u2010infected donors responded against one of these epitopes (p 17.1) irrespective of their serological status relative to antibodies to EA\u2010R. Both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cell subpopulations from seropositive donors proliferated in the presence of p17.1 in short\u2010term cultures. These experiments therefore identified one T\u2010cell epitope on the 17\u2010kDa polypeptide. In contrast, sera from anti\u2010EA antibody\u2010positive individuals reacted with all 3 synthetic peptides to varying degrees, with p 17.1 being the most frequently reactive epitope. When the sera were grouped according to diagnosis, it was noted that 82% of the sera from patients with aggressive lymphomas, whether Africans with Burkitt's lymphoma or North Americans with intermediate\u2010grade large\u2010cell or high\u2010grade B\u2010cell lymphoma, contained antibody reactive with p17.1, while 64% were reactive with p 17.2 and 29% with p 17.3. In contrast, high anti\u2010EA antibody\u2010positive sera from nasopharyngeal carcinoma patients were relatively less reactive with these synthetic peptides (23% positive with p17.1; 19% with p17.2; and 13% with p17.3). These results therefore identified 3 8\u2010cell EA\u2010R epitopes which might be potentially useful for clinical or epidemiological studies of EBV\u2010associated lymphoproliferative diseases.<\/jats:p>","DOI":"10.1002\/ijc.2910530204","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T23:27:34Z","timestamp":1171927654000},"page":"199-204","source":"Crossref","is-referenced-by-count":9,"title":["Identification of T\u2010 and B\u2010cell epitopes associated with a restricted component of the epstein\u2010barr virus\u2010induced early antigen complex"],"prefix":"10.1002","volume":"53","author":[{"given":"Susan","family":"Pothen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tin","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul H.","family":"Levine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Levine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary R.","family":"Pearson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/310207a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198604033141403"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-4508-7_52"},{"key":"e_1_2_1_5_1","first-page":"163","volume-title":"The Epstein\u2010Bav virus, recent advances","author":"Cleary M. 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