{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:21:56Z","timestamp":1760782916315},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4914,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have established 2 Philadelphia chromosome (Ph<jats:sup>1<\/jats:sup>)\u2010positive acute lymphoblastic leukemia (ALL) cell lines, designated PALL\u20101 and PALL\u20102, from distinct adult Ph<jats:sup>1<\/jats:sup>\u2010positive ALL patients. PALL\u20101 was established in nude mice, and PALL\u20102 was established in culture. Both retained the Ph<jats:sup>1<\/jats:sup> chromosome and expressed the ALL type <jats:italic>bcr<\/jats:italic>\/<jats:italic>abl<\/jats:italic> chimeric mRNA containing the junction of the first exon of BCR gene (eI) and second exon of c\u2010<jats:italic>abl<\/jats:italic> gene (a2). PALL\u20101 and PALL\u20102 expressed CD34 surface antigen which is characteristic of early hematopoietic progenitor cells. PALL\u20102 expressed antigens for both pre\u2010B and early myeloid cells and had rearrangements of both the heavy chain of immunoglobulin gene and the \u03b2 chain of T\u2010cell\u2010receptor gene. Both PALL\u20101 and PALL\u20102 expressed detectable levels of <jats:italic>p<\/jats:italic>53 gene RNA. Polymerase\u2010chain\u2010reaction\u2010single\u2010strand conformation polymorphism (PCR\u2010SSCP) analysis of the <jats:italic>p<\/jats:italic>53 gene showed a normal pattern of mobility in both cell lines. Taken together, the 2 cell lines had features of Ph<jats:sup>1<\/jats:sup>\u2010positive ALL (i) hematopoietic progenitor cells with pre\u2010B\u2010cell phenotype and, (ii) activation of el\u2010a2 type <jats:italic>bcr<\/jats:italic>\/<jats:italic>abl<\/jats:italic> oncogene without alterations of <jats:italic>p<\/jats:italic>53 gene. These unique lines should provide a valuable tool for studying the pathogenesis of Ph<jats:sup>1<\/jats:sup>\u2010positive ALL.<\/jats:p>","DOI":"10.1002\/ijc.2910530318","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T23:26:49Z","timestamp":1171927609000},"page":"457-462","source":"Crossref","is-referenced-by-count":9,"title":["Phenotypic and molecular analysis of ph<sup>1<\/sup>\u2010chromosome\u2010positive acute lymphoblastic leukemia cell lines"],"prefix":"10.1002","volume":"53","author":[{"given":"T.","family":"Miyagi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Ohyashiki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Yamato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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