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In order to investigate the mechanism of this deficiency, we have compared group\u20101 BL lines, their sub\u2010lines which have progressed <jats:italic>in vitro<\/jats:italic> towards an LCL\u2010like phenotype (group\u2010III BL), and EBV\u2010transformed lymphoblastoid cell lines (LCLs), for their ability to bind and process tetanus toxoid (TT). The uptake and internalization of <jats:sup>125<\/jats:sup>I\u2010labelled TT was equivalent in the 3 cell types. Only LCLs and group\u2010III BL lines were able to process the IT,as shown by the identification of discrete proteolytic products after separation of whole\u2010cell extracts in tricine\u2010SDS\u2010polyacrylamide gels, and by the recovery of TCA\u2010soluble radioactivity in the culture supernatant. Processing of TT was induced by expression of the EBV\u2010 encoded membrane protein LMPI in transfected group\u2010I BLs. The present findings suggest that the inability of group\u2010I BLs to act as APC is due to their failure to process exogenous antigens. This function appears to be related to phenotypic properties that can be modulated by the expression of LMPI.<\/jats:p>","DOI":"10.1002\/ijc.2910530525","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T23:16:06Z","timestamp":1171926966000},"page":"856-862","source":"Crossref","is-referenced-by-count":25,"title":["Antigen processing and presentation by EBV\u2010carrying cell lines: Cell\u2010phenotype dependence and influence of the EBV\u2010encoded LMP1"],"prefix":"10.1002","volume":"53","author":[{"given":"Pedro O.","family":"de Campos\u2010Lima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigurbj\u00f6rg","family":"Torsteinsd\u00f3ttir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Cuomo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dov","family":"Sulitzeanu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria G.","family":"Masuccii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910400521"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910190105"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830140403"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830201019"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.1.85"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(90)90019-8"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-71-7-1481"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/343133a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)37575-1"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.4049\/jimmunol.129.4.1446","article-title":"Antigen presentation by human B cells: T\u2010cell proliferation induced by Epstein\u2010Barr\u2010virus B\u2010lymphoblas\u2010 toid cells","volume":"129","author":"Issekutz T.","year":"1982","journal-title":"J. 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