{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:23:11Z","timestamp":1791080591225,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4756,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1993,7,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Five continuous cell lines have been established from 29 ocular melanomas and maintained for periods ranging from 3 to 9 years in medium identical to that in which 3 concomitantly studied lines of cutaneous melanoma cells were cultured as controls. The long\u2010term problems to be overcome in establishing uveal cell lines are related to cell\u2010doubling times which ranged from 72 to 432 hr, and plating efficiency, which ranged from 0.5%\u20136.5%. Tumors and cell lines were found to contain melanosomes. The morphology of uveal cells during the early subcultures exhibited multiple changes. Two different established cell lines were obtained from one ciliary\u2010body tumor. Biochemical studies revealed markers of melanogenesis and neuroendocrine compounds. Cytogenetic studies revealed chromosomal abnormalities that differed between uveal and conjunctival melanomas.<\/jats:p>","DOI":"10.1002\/ijc.2910540513","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T23:32:12Z","timestamp":1171927932000},"page":"784-792","source":"Crossref","is-referenced-by-count":36,"title":["Establishment and characterization of human ocular melanoma cell lines"],"prefix":"10.1002","volume":"54","author":[{"given":"Christian","family":"Aubert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Rouge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Reillaudou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Metge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1284","article-title":"Establishment of cell lines of uveal melanoma. Methodology and characteristics","volume":"25","author":"Albert D. M.","year":"1984","journal-title":"Invest. Ophthalmol. vis. Sci."},{"key":"e_1_2_1_3_1","first-page":"288","article-title":"Melanogenesis in cultured human neuroblastomas","volume":"12","author":"Aubert C.","year":"1980","journal-title":"Ann. din. Res."},{"key":"e_1_2_1_4_1","first-page":"1029","article-title":"Tumorigenicity of human malignant melanocytes in nude mice in relation to their differentiation in vitro","volume":"64","author":"Aubert C.","year":"1980","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/jnci\/72.1.3","article-title":"Differentiation and tumorigenicity of human malignant melanocytes in relation to their culture conditions","volume":"72","author":"Aubert C.","year":"1984","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1989.tb00153.x"},{"key":"e_1_2_1_7_1","first-page":"2409","article-title":"Transformation d'un m\u00e9lanome malin in vitro. Etude chromosomique","volume":"280","author":"Berger R.","year":"1975","journal-title":"C.R. Acad. Sci. [D] (Paris)"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(76)90487-6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8635(18)30663-6"},{"key":"e_1_2_1_10_1","first-page":"523","article-title":"The natural course of selected malignant melanomas of the choroid and ciliary body","volume":"12","author":"Curtin V. T.","year":"1974","journal-title":"Mod. Probl. Ophthalmol."},{"key":"e_1_2_1_11_1","volume-title":"La pratique de l'analyse chromosomique","author":"Dutrillaux B.","year":"1981"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1982.01030030995020"},{"key":"e_1_2_1_13_1","first-page":"3957","article-title":"Ultrastructural comparison between cultured and tumor cells of human malignant melanoma","volume":"37","author":"Foa C.","year":"1977","journal-title":"Cancer Res."},{"key":"e_1_2_1_14_1","first-page":"557","article-title":"Quantitative analysis of the Callender classification of uveal melanomas of the choroid and ciliary body","volume":"86","author":"Gamel J. W.","year":"1978","journal-title":"Amer. J. Ophthalmol."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)82058-7"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197901)43:1<294::AID-CNCR2820430142>3.0.CO;2-V"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/0002-9394(75)90528-0","article-title":"Change in cell type of human choroidal malignant melanoma in tissue culture","volume":"80","author":"Irvine A. R.","year":"1975","journal-title":"Amer. J. Ophthalmol"},{"key":"e_1_2_1_18_1","first-page":"873","article-title":"Ultrastructural comparison of spindle A, spindle B, and epithelioid\u2010type cells in uveal malignant melanoma","volume":"11","author":"Iwamoto T.","year":"1972","journal-title":"Invest. Ophthalmol."},{"key":"e_1_2_1_19_1","first-page":"1492","article-title":"S 100 immunophenotypes of uveal and cutaneous melanomas","volume":"31","author":"Kan\u2010Mitchell J.","year":"1990","journal-title":"Invest. Ophthalmol. vis. Sci."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/82.22.1765"},{"key":"e_1_2_1_21_1","first-page":"93","volume-title":"Pigment cell","author":"Rorsman H.","year":"1983"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/56.3.489"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1046172"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910540513","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910540513","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T22:07:51Z","timestamp":1698271671000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910540513"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7,9]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,7,9]]}},"alternative-id":["10.1002\/ijc.2910540513"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910540513","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,7,9]]}}}