{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T18:53:36Z","timestamp":1762541616263},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4673,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1993,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>When cells of fresh Burkitt lymphoma (BL) biopsies are explanted into tissue culture, their survival and growth are greatly dependent on the presence of a feeder layer of irradiated fibroblasts. To investigate the nature of this feeder dependence, we characterized the growth requirements of a panel of Epstein\u2010Barr Virus (EBV)\u2010negative and \u2010positive BL cell lines in both the absence and the presence of feeder cells <jats:italic>in vitro.<\/jats:italic> Four EBV\u2010negative BL lines and 4 EBV\u2010positive lines displaying the phenotype of BL cells <jats:italic>in vivo<\/jats:italic> required high cell density for proliferation in the absence of feeder cells, but grew out as single\u2010cell clones when seeded on irradiated human fibroblasts. EBV\u2010positive BL cell lines which had acquired an activated phenotype similar to that of lymphoblastoid cell lines required much lower cell densities for autonomous proliferation. The EBV\u2010negative Burkitt lymphoma line BL70 was used as a model to study the feeder\u2010cell dependence in more detail. BL70 cells grew in the absence of feeder cells only at high cell density and at high FCS concentration. In the presence of feeder cells, BL70 cells became clonogenic even at greatly reduced FCS concentration. A decrease in either cell density or FCS concentration induced apoptosis. Supernatants from feeder cells and from BL70 cells growing autonomously at high cell density were unable to substitute for the survival and growth\u2010promoting effect of the feeder cells. Protection of Burkitt lymphoma cells from apoptosis by co\u2010cultivation with irradiated fibroblasts was not mediated by induction of bcl\u20102. The system described here may be relevant for the <jats:italic>in vivo<\/jats:italic> situation since Burkitt lymphoma cells are protected from c\u2010MYC induced apoptosis <jats:italic>in vivo<\/jats:italic> also by a mechanism which does not involve bcl\u20102.<\/jats:p>","DOI":"10.1002\/ijc.2910550327","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T23:44:49Z","timestamp":1171928689000},"page":"485-491","source":"Crossref","is-referenced-by-count":24,"title":["Irradiated fibroblasts protect burkitt lymphoma cells from apoptosis by a mechanism independent of BCL\u20102"],"prefix":"10.1002","volume":"55","author":[{"given":"Martin H.","family":"Falk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georg W.","family":"Bornkamm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lothar","family":"H\u00fcltner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Milner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher D.","family":"Gregory","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/358505a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1182\/blood.V75.9.1827.1827","article-title":"Low expression of lymphocyte function\u2010associated antigen (LFA)\u20101 and LFA\u20103 adhesion molecules is a common trait in Burkitt's lymphoma associated with and not associated with Epstein\u2010Barr virus","volume":"75","author":"Billaud M.","year":"1990","journal-title":"Blood"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.5.1613"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.136.3.604"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910510619"},{"key":"e_1_2_1_7_1","first-page":"71","article-title":"In vitro culture of a primary plasmacytoma that has retained its dependence on pristane conditioned microenvironment for growth","volume":"166","author":"Degrassi A.","year":"1990","journal-title":"Curr. 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