{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:16:12Z","timestamp":1771002972827,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4652,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1993,10,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have established an <jats:italic>in vitro<\/jats:italic> angiogenesis model using human omental microvascular endothelial (HOME) cells, in which epidermal growth factor (EGF) or transforming growth factor\u2010alpha (TGF\u2010\u03b1) stimulated cell migration and tube formation. In this study, we examined whether \u03b1\u2010guaiaconic acid (GR\u201012) and its synthetic 20 derivatives showed inhibition of cell migration and tubular formation of HOME cells. We found that GR\u201012 inhibits arachidonic acid metabolism, while GR\u201012 and one derivative, GS\u201001, inhibit tubular formation of endothelial cells in our model system. Confluent monolayers of HOME cells were damaged with a razor blade and incubated with or without TGF\u2010\u03b1; HOME cell migration was stimulated about 1.5\u2010fold over control values in the presence of TGF\u2010\u03b1. Treatment of HOME cells with GR\u201012 or GS\u201001 inhibited both spontaneous and TGF\u2010\u03b1\u2010stimulated migration. GR\u201012 or GS\u201001 inhibited TGF\u2010\u03b1\u2010induced HOME\u2010cell tube formation in type\u20101 collagen gels. We examined whether these compounds could modulate tubular formation of HOME cells induced by human cancer cells. Enhanced tube formation of HOME cells by co\u2010cultured esophageal cancer cells was almost completely inhibited by co\u2010administration of GR\u201012 or GS\u201001. Both compounds also inhibited formation of tubular networks of HOME cells on Matrigels. We also examined anti\u2010angiogenic activity of these compounds in an <jats:italic>in vivo<\/jats:italic> model system of tumor angiogenesis in mice. In this system, GS\u201001 inhibited development of capillary networks at a rate comparable to that of a well\u2010known anti\u2010angiogenic compound, fumagillin, but GR\u201012 did not. The inhibitor of arachidonic acid metabolism is thus expected to modulate tumor angiogenesis.<\/jats:p>","DOI":"10.1002\/ijc.2910550425","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T23:44:15Z","timestamp":1171928655000},"page":"660-666","source":"Crossref","is-referenced-by-count":23,"title":["Anti\u2010angiogenic activity of arachidonic acid metabolism inhibitors in angiogenesis model systems involving human microvascular endothelial cells and neovascularization in mice"],"prefix":"10.1002","volume":"55","author":[{"given":"Ken\u2010Ichi","family":"Ito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Ono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Abe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michihiko","family":"Kuwano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akio","family":"Morimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimitoshi","family":"Kohno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruaki","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Tomita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Sugenoya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Nishihira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116599"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90591-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.4049\/jimmunol.137.6.1985","article-title":"Subcellular distribution of leukotriene C4 binding units in cultured bovine aortic endothelial cells","volume":"137","author":"Chau L. 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