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To characterize the specificity of Pa\u2010G\u201014, immuno\u2010thin\u2010layer chromatography, biochemical, and enzymatic treatment of glycosphingolipid extracts from human pancreas were used. The antibody bound to all blood\u2010group\u2010A substances; \u03b1GaINAc, but not fucose, was essential for reactivity. In ELISA, Pa\u2010G\u201014 also reacted with ovine and bovine submaxillary mucins but not with porcine submaxillary mucin. Binding to ovine submaxillary mucin was resistant to neuraminidase treatment. In solid\u2010phase absorption assays on synthetic carbohydrate structures, Pa\u2010G\u201014 recognized broadly blood group A, Tn and sialyl\u2010Tn. Using immuno\u2010electron\u2010microscopic techniques, reactivity with all Golgi cisternae and mucin droplets of mucous cells in ovine submaxillary gland was demonstrated. All these assays indicate that Pa\u2010G\u201014 shows a novel specificity, since it binds blood group A, Tn and sialyl\u2010Tn, the common structural feature of these epitopes being the presence of a terminal \u03b1GalNAc sugar unit.<\/jats:p>","DOI":"10.1002\/ijc.2910550527","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T23:39:06Z","timestamp":1171928346000},"page":"857-864","source":"Crossref","is-referenced-by-count":3,"title":["\u03b1 galnac is essential for recognition of EXO\u20101 epithelial antigen by mouse monoclonal antibody Pa\u2010G\u201014"],"prefix":"10.1002","volume":"55","author":[{"given":"Helmut","family":"Schr\u00f6der","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Franc\u00ed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"Egea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Oriol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Klingel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang G.","family":"Dippold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco X.","family":"Real","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"2434","article-title":"Gangliosidemediated modulation of cell growth","volume":"261","author":"Bremer E. 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