{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:13:23Z","timestamp":1760955203216},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,7,5]],"date-time":"2007-07-05T00:00:00Z","timestamp":1183593600000},"content-version":"vor","delay-in-days":4932,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> infection is causally related to chronic type\u2010B gastritis, and may also be associated with an increased risk of gastric carcinoma. Vacuolating cytotoxin, which is an 87\u2010kDa protein secreted by <jats:italic>H. pylori<\/jats:italic>, induces eukaryotic cell vacuolation <jats:italic>in vitro.<\/jats:italic> To determine whether there is an association between <jats:italic>H. pylori<\/jats:italic> vacuolating cytotoxin and gastric carcinoma, we investigated several characteristics of <jats:italic>H. pylori<\/jats:italic> infection, <jats:italic>i.e.<\/jats:italic>, isolation of <jats:italic>H. pylori<\/jats:italic> from gastric biopsies, antibodies specific for <jats:italic>H. pylori<\/jats:italic>, detection of neutralizing activity to vacuolating cytotoxin in serum and immunological detection of cytotoxin by serum. Out of 6 sera from gastric\u2010carcinoma patients, all showed the neutralizing activity to vacuolating cytotoxin, in contrast to 3 of 5 sera from peptic\u2010ulcer patients. Normal individuals showed no neutralizing activity. All sera possessing the neutralizing activity recognized an 87\u2010kDa protein band by Western blot analysis. Our results confirmed that cytotoxin\u2010neutralizing activity in human sera was associated with immunodetection of an 87\u2010kDa protein. To further evaluate neutralizing activity in serum from gastric\u2010carcinoma patients, we retrospectively analyzed frozen\u2010stocked serum samples from 22 gastric\u2010carcinoma patients. Sera from 21 of these 22 patients exhibited neutralizing activity. These sera were also checked for antibodies to <jats:italic>H. pylori<\/jats:italic>, using an ELISA; 16 sera showed positive results. Our results indicate that detection of cytotoxin\u2010neutralizing activity in sera is strongly associated with <jats:italic>H. pylori<\/jats:italic> infection, and probably with gastric carcinoma, and is also of interest in the diagnosis of <jats:italic>H. pylori<\/jats:italic> infection. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910560111","type":"journal-article","created":{"date-parts":[[2010,7,7]],"date-time":"2010-07-07T15:27:01Z","timestamp":1278516421000},"page":"56-60","source":"Crossref","is-referenced-by-count":39,"title":["High prevalence of neutralizing activity to helicobacter pylori cytotoxin in serum of gastric\u2010carcinoma patients"],"prefix":"10.1002","volume":"56","author":[{"given":"Masamichi","family":"Hirai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Azuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeji","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuji","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Kohli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Fujiki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,7,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(87)91028-6"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.4.626"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/86.5.575"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-7502-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50054-0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115967"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/iai.58.3.603-610.1990","article-title":"Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity","volume":"58","author":"Cover T. 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