{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T00:41:54Z","timestamp":1700268114180},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4535,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to evaluate whether the SCID mouse can provide the microenvironment for the growth of human immature T\u2010cell leukemias and if <jats:italic>in vivo<\/jats:italic> growth alters their phenotype, we examined the behavior of 3 well\u2010characterized T\u2010cell acute lymphoblastic leukemia (T\u2010ALL)\u2010derived T\u2010cell lines which are at different stages of maturation (CEM, SUP\u2010T3 and MOLT\u20104f) after transfer to non\u2010irradiated SCID mice. All 3 T\u2010cell lines engrafted and proliferated to form tumors in the mice and showed dissemination patterns in the SCID mouse comparable to those of T\u2010ALL in man: <jats:italic>i.e.<\/jats:italic>, human cells were detectable by flow cytpmetry or were cultured from mouse bone marrow, spleen, liver or thymus. CEM, which is the most immature T\u2010cell line, readily formed tumors after injection of cells. The more mature T\u2010cell lines, SUP\u2010T3 and MOLT\u20104f, required a longer time period, even after injection of higher cell numbers. Whereas no changes in the configuration of the rearranged T\u2010cell receptor genes were detected, striking phenotypic changes were observed in all 3 leukemias growing in the SCID mice after injection. SCID\u2010CEM cells showed an increase in the surface expression of CD3 and CDS and a decrease in the expression of CD1 and CD71 (transferrin\u2010receptor). SCID\u2010MOLT\u20104f cells showed an increase in CD5 and CD5 expression and a decrease in CD45RA expression. SCID\u2010SUP\u2010T3 cells showed increased expression of CD5 and CD45RA. Apparently, the mouse environment caused changes in cell\u2010surface antigen expression on the T\u2010ALL\u2010derived T\u2010cell lines. Some immunophenotypic changes remained stable during subsequent growth in culture of SUP\u2010T3 cells, suggesting that maturation of the cell line occurred <jats:italic>in vivo.<\/jats:italic> The other cell lines, CEM and MOLT\u20104f after undergoing <jats:italic>in vivo<\/jats:italic>\u2010induced changes, reverted to the original immunopheno\u2010type, suggesting transitory activation <jats:italic>in vivo.<\/jats:italic> These data point out the importance of stromal factors in defining growth and maturation of human leukemic cells <jats:italic>in vivo<\/jats:italic>, in SCID mice.<\/jats:p>","DOI":"10.1002\/ijc.2910560414","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:14:06Z","timestamp":1171930446000},"page":"546-551","source":"Crossref","is-referenced-by-count":7,"title":["The scid mouse environment causes immunophenotypic changes in human immature T\u2010cell lines"],"prefix":"10.1002","volume":"56","author":[{"given":"Christel H.","family":"Uittenbogaart","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah J.","family":"Anisman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"Tary\u2010Lehmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leanne W.","family":"Vollger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo M.","family":"Breit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques J. 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