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The Wag rat colon carcinoma CC531 was injected via a tail vein to induce pulmonary metastases. Syngeneic A\u2010NK cells were labeled with the fluorescent dye rhodamine (TRITC) and next injected via a tail vein in rats bearing day\u201012 lung tumors. The number of A\u2010NK cells in tumor and in normal tissue per rat was counted in sections after administration of A\u2010NK cells. At all time points tested, a significant linear relationship between the cross\u2010section area of the tumor and the number of infiltrating cells was observed, but small tumor areas became fully infiltrated earlier than larger areas. At 24 hr after injection, approximately 10% of the injected cells were found in the tumor tissue and the average A\u2010NK\u2010cell\u2010to\u2010tumor\u2010cell ratio was estimated to be 1:3. A\u2010NK cells were found in the liver too, although the number of cells per mm<jats:sup>2<\/jats:sup> tissue was low compared with the pulmonary tumor tissue. Very low numbers of A\u2010NK cells were found in kidney, adrenal gland, spleen, and blood. We conclude that, in this syngeneic rat model, adoptively transferred A\u2010NK cells are able to find and specifically infiltrate pulmonary metastases in a time\u2010dependent fashion.<\/jats:p>","DOI":"10.1002\/ijc.2910560418","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:14:06Z","timestamp":1171930446000},"page":"574-579","source":"Crossref","is-referenced-by-count":23,"title":["The infiltration of experimentally induced lung metastases of colon carcinoma CC531 by adoptively transferred interleukin\u20102\u2010activated natural killer cells in wag rats"],"prefix":"10.1002","volume":"56","author":[{"given":"Peter J. 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