{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:55:19Z","timestamp":1760954119370},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4507,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Despite the steadily increasing number of patients suffering from squamous\u2010cell carcinomas of the oropharyngeal region, little is known about the molecular steps involved in the induction of these neoplasms. We investigated oropharyngeal cancers from 38 patients for mutations in the <jats:italic>p53<\/jats:italic> tumour\u2010suppressor gene. The majority of patients (74%) had a history of tobacco and alcohol abuse. Five had lymph\u2010node metastases, 3 had multiple primary carcinomas and 2 presented with multiple primary tumours and lymph\u2010node metastases. Exons 5 through 8 of the <jats:italic>p53<\/jats:italic> gene were screened by single\u2010strand conformation polymorphism analysis followed by direct DNA sequencing. A total of 16 tumours (42%) contained point mutations which were scattered throughout exons 5 to 8. Most mutations (56%) were transitions, predominantly G\u2192A. Among the transversions, G\u2192T mutations prevailed; these have also been found in smoking\u2010related lung cancer. One carcinoma of the soft palate showed a mutation which was retained in a lymph\u2010node metastasis. In another patient, 2 primary carcinomas had different mutations, indicating that they had developed independently. Similar results were obtained in a case with a <jats:italic>p53<\/jats:italic> mutation in the third of 3 primary tongue carcinomas which developed over a period of 23 years. One lymph\u2010node metastasis had a 12\u2010bp deletion which was not detected in any of the primary malignancies. The frequent occurrence of <jats:italic>p53<\/jats:italic> mutations in oropharyngeal carcinomas supports the view that they play a role in the initiation or progression of the malignant phenotype.<\/jats:p>","DOI":"10.1002\/ijc.2910560608","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:10:13Z","timestamp":1171930213000},"page":"807-811","source":"Crossref","is-referenced-by-count":41,"title":["<i>p53<\/i> gene mutations in oropharyngeal carcinomas: A comparison of solitary and multiple primary tumours and lymph\u2010node metastases"],"prefix":"10.1002","volume":"56","author":[{"given":"Maimoona","family":"Zariwala","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madeleine","family":"Pfaltz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Ohgaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Kleihues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhold","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"4832","article-title":"Occurrence of p53 gene deletions and human papilloma virus infection in human head and neck cancer","volume":"52","author":"Brachman D. 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