{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:24:06Z","timestamp":1791397446596,"version":"4.3.3"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":4477,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human interleukin\u201010 (h\u2010IL\u201010) is a pleiotropic cytokine with stimulatory activity on B\u2010Iymphocytes. Recent evidence indicates that infection with Epstein\u2010Barr virus (EBV) induces h\u2010IL\u201010 production in B\u2010cells and that this cytokine may contribute to EBV\u2010induced B\u2010cell transformation. It is not known whether h\u2010IL\u201010 induction by EBV correlates with distinct phenotypic features of the infected cells or with the expression of particular viral genes. We have approached these questions by investigating the expression of h\u2010IL\u201010 mRNA in a panel of B\u2010cell lines including: in vitro EBV\u2010transformed lymphoblastoid cell lines (LCLs), EBV\u2010carrying Burkitt lymphoma (BL) lines, EBV\u2010negative BL lines and their sublines infected with different EBV strains, or transfected with the transformation\u2010associated viral gene. h\u2010IL\u201010 mRNA was detected by reverse\u2010transcriptase\u2010assisted (RT)\u2010PCR in a subset of EBV\u2010negative BLs and in all FBV\u2010positive BL lines and LCLs investigated except Daudi. This cell line carries an EBV nuclear antigen (EBNA)\u20102 gene\u2010defective virus strain. h\u2010IL\u201010 mRNA was induced by conversion of 3 EBV\u2010negative and h\u2010IL\u201010\u2010negative BL lines (BL41, BL47 and BL49) with the transforming, B95.8\u2010derived EBV strain. P3HR\u20101 virus convertants that do not express the viral EBNA\u20102 and the EBV latent membrane protein (LMP)\u20101, and fail to progress towards a LCL\u2010like cell phenotype, showed no evidence of h\u2010IL\u201010 up\u2010regulation. Expression of LMP1 was sufficient to induce h\u2010IL\u201010 mRNA in transfected sublines of the EBV\u2010negative DG75 and BL41 cell lines, whereas expression of EBNA1, 2, 5 or 6 had no effect. h\u2010IL\u201010 was detected in the culture supernatants of the LMP1 transfectants by specific ELISA assays. The present findings confirm the role of LMP1 in the transactivation of a wide variety of cellular genes which may be involved in EBV\u2010induced B\u2010cell transformation. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910570218","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:09:48Z","timestamp":1171930188000},"page":"240-244","source":"Crossref","is-referenced-by-count":120,"title":["The epstein\u2010barr virus latent membrane protein\u20101 (LMP1) induces interleukin\u201010 production in burkitt lymphoma lines"],"prefix":"10.1002","volume":"57","author":[{"given":"Hiroshi","family":"Nakagomi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Dolcetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Bejarano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Pisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Kiessling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria G.","family":"Masucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1128\/jvi.64.5.2126-2134.1990","article-title":"Epstein\u2010Barr virus nuclear antigen 2 (EBNA2) induces expression of the virus\u2010coded latent membrane protein (LMP)","volume":"64","author":"Abbot S. D.","year":"1990","journal-title":"J. Virol."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1992.tb01406.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/4.12.1389"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1182\/blood.V80.5.1289.1289","article-title":"Human B\u2010cell interleukin\u201010: B\u2010cell lines derined from patients with acquired immunodeficiency syndrome and Burkitt's lymphomas constitutively secrete large quantities of interlekin\u201010","volume":"80","author":"Benjamin D.","year":"1992","journal-title":"Blood"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1128\/jvi.43.3.952-968.1982","article-title":"Deletion of the non\u2010transforming Epstein\u2010Barr virus strain P3HR\u20101 causes fusion of the large internal repeat to the DSL region","volume":"43","author":"Bornkamm G. W.","year":"1982","journal-title":"J. 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Identification of restriction enzyme fragments which contain DNA sequences which differ among strains of EBV","volume":"27","author":"Raab\u2010Traub N.","year":"1978","journal-title":"J. Virol."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.5.1890"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910350404"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02568.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.4.1172"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1128\/jvi.62.11.4173-4184.1988","article-title":"Epstein\u2010Barr virus latent infection membrane protein alters the human B\u2010lymphocyte phenotype: deletion of the amino terminus abolishes activity","volume":"61","author":"Wang D.","year":"1988","journal-title":"J. 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