{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:07:08Z","timestamp":1789560428407,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4460,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cyclin D1 is a cell\u2010cycle regulator essential for G<jats:sub>1<\/jats:sub>, phase progression and a candidate proto\u2010oncogene implicated in pathogenesis of several human tumour types, including breast carcinomas. In spite of the accumulating genetic evidence, however, there are no data regarding abundance and properties of the cyclin D1 protein in breast cancer. We now report aberrant nuclear overexpression\/accumulation of the cyclin D1 protein in about half of the 170 primary breast carcinoma specimens analyzed by monoclonal antibody immunohistochemistry, indicating that the frequency of cyclin D1 abnormalities may be considerably higher than previously deduced from DNA amplification studies. A comparison of the expression patterns in matched lesions at different stages of tumour progression revealed that the cyclin D1 protein aberration appears to reflect a relatively early event and that, when acquired by a tumour, it is maintained throughout breast cancer progression including metastatic spread. In both tumour tissues and breast cancer cell lines, the abundance of this protein shows characteristic variations consistent with a cell\u2010cycle oscillation and the peak levels expressed in G<jats:sub>1<\/jats:sub>. In all 7 cell lines whose retinoblastoma (Rb) protein is mutant or complexed to SV40 T antigen, exceptionally low levels of cyclin D1 protein and mRNA were found. Antibody\u2010mediated and anti\u2010sense oligonucleotide knockout experiments demonstrate the requirement for the cell\u2010cycle regulatory function of cyclin D1 in breast cancer lines with single or multiple copies of the gene and reveal the absence of such a requirement in the cell lines with Rb defects. Our data are consistent with the notion that the emerging \u201cRb\u2010cyclin D1 pathway\u201d represents a frequent target of oncogenic abnormalities in breast cancer. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910570311","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:09:23Z","timestamp":1171930163000},"page":"353-361","source":"Crossref","is-referenced-by-count":413,"title":["Cyclin D1 protein expression and function in human breast cancer"],"prefix":"10.1002","volume":"57","author":[{"given":"Jirina","family":"Bartkova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Lukas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heiko","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorrit","family":"L\u00fctzh\u00f8t","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Strauss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Bartek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.7.5.812"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.9.3520"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910450620"},{"issue":"1","key":"e_1_2_1_5_1","first-page":"51","article-title":"Molecular pathology of the cell cycle in human cancer cells","volume":"11","author":"Bartek J.","year":"1993","journal-title":"Stem Cells."},{"key":"e_1_2_1_6_1","first-page":"101","article-title":"Cellular localization and T antigen binding of the retinoblastoma protein","volume":"7","author":"Bartek J.","year":"1992","journal-title":"Oncogene"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(05)80431-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","unstructured":"Bartkova J. 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