{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:02:04Z","timestamp":1790488924266,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4446,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies have shown that molecules involved in generation and regulation of extracellular proteolytic activity are often expressed by non\u2010malignant stromal cells during human cancer invasion. We have studied the expression of the urokinase\u2010type plasminogen activator and the urokinase\u2010type plasminogen activator cell\u2010surface receptor in xenografts of human MDA\u2010MB\u2010231 mammary carcinoma cells growing invasively in nude mice. Northern analysis showed the presence of both human and mouse urokinase\u2010type plasminogen activator and urokinase\u2010type plasminogen activator receptor mRNA in tumor extracts. By <jats:italic>in situ<\/jats:italic> hybridization, mRNA for human urokinase\u2010type plasminogen activator and its receptor was detected in virtually all the cancer cells, while mouse urokinase\u2010type plasminogen activator and urokinase\u2010type plasminogen activator receptor mRNA was expressed by tumor\u2010infiltrating fibroblast\u2010like and macrophage\u2010like cells. In invasive areas the cells expressing the 2 murine mRNAs were either the same or located immediately adjacent to each other. This model system has several advantages for studies of the mechanism by which cancer cells induce or recruit stromal cells to produce molecules involved in proteolysis. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910570419","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:06:02Z","timestamp":1171929962000},"page":"553-560","source":"Crossref","is-referenced-by-count":48,"title":["Expression of uPA and its receptor by both neoplastic and stromal cells during xenograft invasion"],"prefix":"10.1002","volume":"57","author":[{"given":"John","family":"R\u00f8mer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Pyke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leif R.","family":"Lund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Eriksen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kristensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebbe","family":"R\u00f8nne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunilla","family":"H\u00f8yer\u2010Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keld","family":"Dan\u00f8","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils","family":"Br\u00fcnner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(90)90164-4"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(92)90245-W"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.11.5021"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60028-7"},{"key":"e_1_2_1_6_1","volume-title":"Proteolysis and protein turnover","author":"Dan\u00f8 K.","year":"1994"},{"key":"e_1_2_1_7_1","volume-title":"Fibrinolysis in disease","author":"Ellis V.","year":"1994"},{"key":"e_1_2_1_8_1","first-page":"111","article-title":"Localization of urokinase\u2010type plasminogen activator in stromal cells in adenocarcinomas of the colon in humans","volume":"138","author":"Grondahl\u2010Hansen J.","year":"1991","journal-title":"Amer. J. Pathol."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910490507"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.6.1763"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"3399","DOI":"10.1002\/j.1460-2075.1991.tb04904.x","article-title":"Urokinase\u2010receptor biosynthesis, messenger RNA level and gene transcription are increased by transforming growth factor\u2010beta 1 in human A549 lung carcinoma cells","volume":"1","author":"Lund L. R.","year":"1991","journal-title":"EMBO J."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2437"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2437"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1083\/jcb.115.4.1107","article-title":"In vivo paracrine interaction between urokinase and its receptor\u2014effect on tumour cell invasion","volume":"115","author":"Ossowski L.","year":"1991","journal-title":"J. cell Biol."},{"key":"e_1_2_1_15_1","first-page":"274","article-title":"Inhibition of urokinase\u2010type plasminogen activator by antibodies\u2014the effect on dissemination of a human tumour in the nude mouse","volume":"51","author":"Ossowski L.","year":"1991","journal-title":"Cancer Res."},{"key":"e_1_2_1_16_1","first-page":"389","article-title":"Stromal expression of 72 kDa type\u2010IV collagenase (MMP\u20102) and TIMP\u20102 messenger RNAs in colorectal neoplasia","volume":"141","author":"Poulson R.","year":"1992","journal-title":"Amer. J. Pathol."},{"key":"e_1_2_1_17_1","first-page":"1911","article-title":"Receptor for urkinase is present in tumor\u2010associated macrophages in ductal breast carcinoma","volume":"53","author":"Pyke C.","year":"1993","journal-title":"Cancer Res."},{"key":"e_1_2_1_18_1","first-page":"1336","article-title":"Localization of messenger RNA for Mr 72,000 and 92,000 type\u2010IV collagenases in human skin cancers by in situ hybridization","volume":"52","author":"Pyke C.","year":"1992","journal-title":"Cancer Res."},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","unstructured":"Pyke C. Ralfki\u00e6r E. Ronne E. H\u00f8yer\u2010Hansen G. Kirkeby L.andDan\u00f8 K. Immunohistochemical detection of the receptor for urokinase plasminogen activator in human colon cancer.Histopathology (1994). (In press).","DOI":"10.1111\/j.1365-2559.1994.tb01291.x"},{"key":"e_1_2_1_20_1","first-page":"359","article-title":"Messenger RNA for two type IV collagenases is located in non\u2010malignant stromal cells in human colon cancer","volume":"142","author":"Pyke C.","year":"1993","journal-title":"Amer. J. Pathol."},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1091\/mbc.2.10.793","article-title":"Complementation between urokinase\u2010producing and receptor\u2010producing cells in extracellular matrix degradation","volume":"2","author":"Quax P. H. A.","year":"1991","journal-title":"Cell Regul."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb08132.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12486833"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115406"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1992.tb16799.x"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.5.1843"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910570419","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910570419","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T19:55:33Z","timestamp":1698177333000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910570419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5,15]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1994,5,15]]}},"alternative-id":["10.1002\/ijc.2910570419"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910570419","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5,15]]}}}