{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T10:47:02Z","timestamp":1698144422342},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4429,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adult T\u2010cell leukemia\/lymphoma (ATLL) is a malignancy involving peripheral blood, lymph nodes, skin and other organs. Human T\u2010cell leukemia virus type 1 (HTLV\u20101) is etiologically associated with ATLL but cannot be detected by conventional methods in fresh samples of peripheral blood and skin taken from ATLL. The aim of this study was to investigate the feasibility of an <jats:italic>in situ<\/jats:italic> hybridization technique for detection of HTLV\u20101 mRNA in atypical lymphoid cells of peripheral blood and skin lesions of patients with ATLL. We detected variable amounts of HTLV\u20101 <jats:italic>tax<\/jats:italic> mRNA in the nuclei and cytoplasm of these cells in fresh peripheral blood samples and skin lesions from ATLL patients, and also in asymptomatic HTLV\u20101 infected donors to a lesser extent. Out of 10 patients with ATLL, 7 showed strong positive <jats:italic>in situ<\/jats:italic> hybridization whereas the other 3 were only weakly positive. However, in the last 3 cases, the reaction became strongly positive after cells had been cultured for 24 hr. Furthermore, all 3 asymptomatic HTLV\u20101\u2010infected donors exhibited a weakly positive response in their apparently mature lymphoid cells.<\/jats:p>","DOI":"10.1002\/ijc.2910570525","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:05:22Z","timestamp":1171929922000},"page":"760-764","source":"Crossref","is-referenced-by-count":9,"title":["HTLV\u2010I messenger RNA is expressed <i>in vivo<\/i> in adult T\u2010cell leukemia\/lymphoma patients: An <i>in situ<\/i> hybridization study"],"prefix":"10.1002","volume":"57","author":[{"given":"Mitsuru","family":"Setoyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshinobu","family":"Fujiyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shimako","family":"Mizoguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Katahira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Yashiki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsutoshi","family":"Tara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamotsu","family":"Kanzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunro","family":"Sonoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890330113"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-372180-8.50043-3"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.19.6207"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1128\/jvi.65.3.1628-1633.1991","article-title":"Human T\u2010cell leukemia\u2010lymphoma virus type I (HTLV\u20101) expression in peripheral blood mononuclear cells from patients with tropical spastic paraparesis\/HTLV\u20101\u2010associated myelopathy","volume":"65","author":"Gessain A.","year":"1991","journal-title":"J. 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