{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:57:05Z","timestamp":1761058625787},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":4400,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A 58\u2010year\u2010old Chinese man presented initially with generalized lymphadenopathy, and lymph\u2010node biopsy showed disturbed architecture with preponderance of large B\u2010blasts mixed with numerous CD8<jats:sup>+<\/jats:sup> T lymphocytes, consistent with an acute Epstein\u2010Barr virus(EBV) infection. Immunohistological and gene rearrangement studies confirmed the absence of clonal T or B cells. Polyclonal EBV with lytic infection was detected by Southern blot hybridization (SoBH). Expression of EBV proteins (EBNA2, LMP and ZEBRA) was detected in a proportion of cells by immunostaining. EBV\u2010lytic proteins EA\u2010D, VCA, MA were also detected in rare scattered cells. Double immunostaining showed that the LMP\u2010positive cells were of B and of T phenotype: 73% CD19<jats:sup>+<\/jats:sup>, 26% CD2<jats:sup>+<\/jats:sup> 23% CD3<jats:sup>+<\/jats:sup> 8% CD4<jats:sup>+<\/jats:sup> 17% CD8<jats:sup>+<\/jats:sup>. After biopsy, there was spontaneous regression of lymph\u2010node enlargement, but lymphadenopathy recurred 8 months later, and the second lymph\u2010node biopsy showed T\u2010cell lymphoma, confirmed by detection of clonally rearranged T\u2010cell\u2010receptor beta\u2010chain gene. However, EBV genome could not be detected in the second biopsy by SoBH, <jats:italic>in situ<\/jats:italic> hybridization for EBV\u2010encoded EBER RNA, and immunostaining for EBNA2, LMP and ZEBRA was also negative. This case is of special interest because an EBV\u2010negative T\u2010cell lymphoma developed shortly after an acute episode of EBV\u2010related lymphoproliferation, even though many EBV\u2010positive T cells were detected during the acute episode. EBV was apparently not a direct cause of the lymphoma, but the close temporal association of the 2 lesions supports the hypothesis that EBV can act as a co\u2010factor in lymphomagenesis. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910580107","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T23:57:40Z","timestamp":1171929460000},"page":"33-39","source":"Crossref","is-referenced-by-count":10,"title":["Epstein\u2010barr virus (EBV)\u2010related lymphoproliferative disorder with subsequent EBV\u2010negative T\u2010cell lymphoma"],"prefix":"10.1002","volume":"58","author":[{"given":"Qian","family":"Tao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gopesh","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shee Loong","family":"Loke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond H. 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