{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:12:33Z","timestamp":1760242353612},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":4355,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have analysed the <jats:italic>p53<\/jats:italic> status in non\u2010progressive and progressive chronic B\u2010cell leukaemia (B\u2010CLL) by ELISA, immunoprecipitation, FACS and cDNA sequencing in relation to <jats:italic>in vitro<\/jats:italic> proliferation in response to <jats:italic>Staphylococcus aureus<\/jats:italic> strain Cowan I (SAC) and IL\u20102. In FACS, cells from progressive leukaemia were found to over\u2010express p53 with a conformation recognized by PAb240. In a PAb240\u2010based ELISA, 60% of progressive B\u2010CLL were positive. DNA sequencing of <jats:italic>p53<\/jats:italic> exons 5 to 9 revealed a codon 179 His to Gln change in one of the ELISA\u2010positive, progressive B\u2010CLL but failed to reveal any mutations in 4 other ELISA\u2010positive, progressive B\u2010CLL Among progressive B\u2010CLL populations, 10\/14 responded by proliferation <jats:italic>in vitro<\/jats:italic> to SAC\/IL\u20102. In non\u2010progressive cells, low levels of p53 were found by FACS, none was positive in the PAb240 ELISA and only one case showed a weak proliferative response to SAC\/IL\u20102. Low p53 expression was also seen in different types of normal B cells, resting and activated, and in EBV\u2010transformed B\u2010cell lines, in contrast to the high expression observed in Burkitt lymphoma cell lines with verified <jats:italic>p53<\/jats:italic> mutations. We conclude that progressive B\u2010CLL is characterized by aberrant <jats:italic>p53<\/jats:italic> expression which may be a significant prognostic factor.<\/jats:p>","DOI":"10.1002\/ijc.2910580403","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:20:36Z","timestamp":1171930836000},"page":"474-479","source":"Crossref","is-referenced-by-count":18,"title":["Progressive B\u2010cell chronic lymphocytic leukaemia frequently exhibits aberrant <i>p53<\/i> expression"],"prefix":"10.1002","volume":"58","author":[{"given":"Miguel","family":"Aguilar\u2010Santelises","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristinn P.","family":"Magnusson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klas G.","family":"Wiman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e5kan","family":"Mellstedt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikael","family":"Jondal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"Aguilar\u2010Santelises M. Mellstedt H.andJondal M. Leukemic cells from progressive B\u2010CLL respond strongly to growth stimulation in vitro. Leukemia (1994) (In press)."},{"key":"e_1_2_1_3_1","first-page":"893","article-title":"Genetic and immunochemical analysis of mutant p53 in human breast cancer cell lines","volume":"5","author":"Bartek J.","year":"1990","journal-title":"Oncogene"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0065-2571(88)90028-3"},{"key":"e_1_2_1_5_1","first-page":"71","article-title":"Recent advances in the treatment of B\u2010cell chronic lymphocytic leukaemia","volume":"4","author":"Cheson B. 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C.","year":"1992","journal-title":"Cancer Res."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V46.2.219.219"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1992.352"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/355846a0"},{"key":"e_1_2_1_22_1","first-page":"1635","article-title":"Human wild\u2010type p53 adopts a unique conformational and phosphorylation state in vivo during growth arrest of glioblastoma cells","volume":"7","author":"Ullrich S. 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