{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T05:00:25Z","timestamp":1770699625215,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4354,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Identification of genetic structure and diversity of T\u2010cell receptor (TCR)\u03b1 and \u03b2 genes for cytotoxic T lymphocytes (CTLs) infiltrating human cancers is important for the better understanding of molecular mechanisms of host defense at tumor sites. cDNAs of TCR\u03b1 and \u03b2 genes of 22 different melanoma\u2010specific CTL clones established from the tumor\u2010infiltrating lymphocytes of 2 patients were sequenced for analysis of their genetic structure and diversity. V\u03b17.2\u2010J\u03b110\u2010C\u03b1 was found in 4 of 22 clones, 2 of which also used the same \u03b2\u2010chain. The other 20 clones showed different combinations of \u03b1 and \u03b2 use. At deduced amino\u2010acid levels, 7 of 9 clones from one patient used a threonine residue at the 26th position in the complementarity\u2010determining region (CDR)1 of TCR\u03b1. Eight of 13 clones used a threonine at the 99th or a serine residue at the 100th position in CDR3 of TCR\u03b1. CTL clones with the same or different TCR\u03b1 showed the same or different patterns of cytotoxicity, respectively. These results suggest that CTLs usually do not demonstrate clonal expansion at tumor sites of metastatic melanoma's but rather that polyclonal T cells capable of binding to multiple melanoma determinants through CDR3 of TCR\u03b1 accumulate in the tumor.<\/jats:p>","DOI":"10.1002\/ijc.2910580407","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:20:36Z","timestamp":1171930836000},"page":"497-502","source":"Crossref","is-referenced-by-count":3,"title":["Polyclonal uses of T\u2010cell receptor (TCR)\u03b1 and \u03b2 genes for cytotoxic T lymphocytes in human metastatic melanoma: possible involvement of TCR\u03b1 in tumor\u2010cell recognition"],"prefix":"10.1002","volume":"58","author":[{"given":"Dai","family":"Seito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Morita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Masuoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideyuki","family":"Saya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyogo","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.4.989"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"4520","DOI":"10.4049\/jimmunol.142.12.4520","article-title":"Human tumor\u2010infiltrating lymphocytes. 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