{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T21:36:57Z","timestamp":1783633017100,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4293,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The expression and cell\u2010membrane distribution of the \u03b2<jats:sub>1<\/jats:sub> family of integrins (very\u2010late\u2010activation antigens, VLA) were investigated in benign and malignant human thyroid tumors. We compared tissue samples of normal glands, nodular goiters, adenomas and carcinomas. We also examined 3 thyroidcarcinoma cell lines cultured <jats:italic>in vitro.<\/jats:italic> The expression of subunits of the \u03b2<jats:sub>1<\/jats:sub> family of integrins was assessed by flow cytometry and specific antibodies in dispersed single\u2010cell suspensions and by immunofluorescence on frozen tissue sections. In contrast to the heterogeneity of the expression of \u03b2<jats:sub>1<\/jats:sub> integrins observed in other tumors, thyroid neoplastic lesions showed a remarkably constant VLA profile. In all tumors, benign as well as malignant, and in carcinoma cell lines, all sub\u2010units of \u03b2<jats:sub>1<\/jats:sub> integrins were expressed at high levels. While sub\u2010units \u03b1<jats:sub>1<\/jats:sub>, \u03b1<jats:sub>3<\/jats:sub>, \u03b1<jats:sub>5<\/jats:sub>, \u03b1<jats:sub>6<\/jats:sub> and occasionally \u03b1<jats:sub>2<\/jats:sub> were also present in a cell sub\u2010set of normal glands and nodular goiters, expression of \u03b1<jats:sub>4<\/jats:sub> was restricted to neoplastic lesions; this integrin can be therefore considered an antigen associated with thyroid tumors. It has been reported that in normal glands and in nodular goiters, the expression of \u03b2<jats:sub>1<\/jats:sub> integrins is restricted to the basal\u2010cell membrane. Immunofluorescence on tissue sections showed instead that, in adenomas and carcinomas, the polarized distribution of these integrins on the cell membrane is lost.<\/jats:p>","DOI":"10.1002\/ijc.2910590208","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:17:20Z","timestamp":1171930640000},"page":"185-190","source":"Crossref","is-referenced-by-count":14,"title":["Loss of polarity and <i>De novo<\/i> expression of the \u03b2<sub>1<\/sub> family of integrins in thyroid tumors"],"prefix":"10.1002","volume":"59","author":[{"given":"Mario","family":"Vitale","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Bassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maddalena","family":"Illario","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Fenzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adele","family":"Casamassima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.6.3455"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910460330"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.24.9238"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2011740"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.5.1111"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90661-W"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02899078"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.1.224"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1697696"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90715-Q"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00049409"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(88)91280-7"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61504-2"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"3011","DOI":"10.4049\/jimmunol.132.6.3011","article-title":"Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T\u2010cell lines","volume":"132","author":"Hemler M. 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