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In this study we have examined the functional importance of these findings by testing the presence of IGF\u2010I in 15 leiomyoma biopsies and in 18 myometrium biopsies and the capacity of smooth\u2010muscle cells cultured from these tissues to react to IGF\u2010I. The mean IGF\u2010I peptide concentration in leiomyomas was 3 times higher than in myometrium. This resulted from increased IGF\u2010I uptake in leiomyomas rather than from increased synthesis, as these tissues contain higher concentrations of type\u2010IIGF receptors, as detected by immunohistochemistry, and equal levels of IGF\u2010I mRNA. Blocking IGF\u2010I transport with cytochalasin\u2010B and with the type\u2010I IGF receptor blocking antibody \u03b1IR3 in cultured cells induced decreased immunostaining intensity for IGF\u2010I in most myometrium and leiomyoma cultures, indicating that the detected IGF\u2010I is internalized. Depending on the culture conditions, IGF\u2010I administration yielded increased survival or a higher proliferation rate in leiomyoma cultures than in myometrium cultures, indicating the increased importance of exogenous IGF\u2010I for the growth of transformed smooth\u2010muscle cells. We conclude that the increased concentrations of type\u2010I IGF receptors in leiomyoma compared to myometrial smooth\u2010muscle cells are functional with respect to the enhanced internalization of IGF\u2010I and that they provide these tumor cells with a growth advantage compared to their normal counterparts. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910590323","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:16:36Z","timestamp":1171930596000},"page":"427-434","source":"Crossref","is-referenced-by-count":21,"title":["Growth advantage of human leiomyoma cells compared to normal smooth\u2010muscle cells due to enhanced sensitivity toward insulin\u2010like growth factor I"],"prefix":"10.1002","volume":"59","author":[{"given":"Leo T. 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